CAKI-1
CAKI-1
规格:
货期:
编号:TS260202
品牌:Testobio
| 产品名称： | CAKI-1 |
|---|---|
| 商品货号： | TS260202 |
| <strong>Cell line </strong>： | CAKI-1 |
| DSMZ no. ： | TS260202 |
| Species ： | human (Homo sapiens) |
| Cell type ： | kidney carcinoma |
| Origin ： | established in 1971 from the cutaneous metastasis of the kidney adenocarcinoma of a 49-year-old man |
| Reference(s) ： | 14538 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in monolayers; image; image; image |
| Medium ： | 80% McCoy´s 5a + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2 flask; split culture 1:2 to 1:4 every 3-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-60 hours |
| Harvest ： | cell harvest of ca. 8-14 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | CAKI-1 |
|---|---|
| 商品货号： | TS260202 |
| <strong>Cell line </strong>： | CAKI-1 |
| DSMZ no. ： | TS260202 |
| Species ： | human (Homo sapiens) |
| Cell type ： | kidney carcinoma |
| Origin ： | established in 1971 from the cutaneous metastasis of the kidney adenocarcinoma of a 49-year-old man |
| Reference(s) ： | 14538 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in monolayers; image; image; image |
| Medium ： | 80% McCoy´s 5a + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2 flask; split culture 1:2 to 1:4 every 3-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-60 hours |
| Harvest ： | cell harvest of ca. 8-14 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
