| 产品名称: | MHH-TALL-2 |
|---|---|
| 商品货号: | TS260207 |
| <strong>Cell line </strong>: | MHH-TALL-2 |
| DSMZ no. : | TS260207 |
| Species : | human (Homo sapiens) |
| Cell type : | T cell acute lymphoblastic leukemia |
| Origin : | established in 1994 from the peripheral blood of a boy with T cell acute lymphoblastic leukemia (T-ALL) |
| Reference(s) : | 16747 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | single cells growing in suspension; image; image |
| Medium : | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture : | seed out at ca. 1-1.5 x 106 cells/ml in 24-well plates; maintain at more than 1 x 106 cells/ml; split saturated culture 1:2 every 3 days; cells are difficult to culture; possibly no cell growth during the first week after thawing |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 60-72 hours |
| Harvest : | maximum density at ca. 3 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in microbiological culture, PCR assays |
| Immunology : | CD2 +, CD3 +, CD4 -, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta +, TCRgamma/delta -; image |
| Fingerprint : | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics : | human diploid karyotype with 2% polyploidy; 46(44-46)<2n>XY; no consistent abnormality detected |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| 产品名称: | MHH-TALL-2 |
|---|---|
| 商品货号: | TS260207 |
| <strong>Cell line </strong>: | MHH-TALL-2 |
| DSMZ no. : | TS260207 |
| Species : | human (Homo sapiens) |
| Cell type : | T cell acute lymphoblastic leukemia |
| Origin : | established in 1994 from the peripheral blood of a boy with T cell acute lymphoblastic leukemia (T-ALL) |
| Reference(s) : | 16747 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | single cells growing in suspension; image; image |
| Medium : | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture : | seed out at ca. 1-1.5 x 106 cells/ml in 24-well plates; maintain at more than 1 x 106 cells/ml; split saturated culture 1:2 every 3 days; cells are difficult to culture; possibly no cell growth during the first week after thawing |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 60-72 hours |
| Harvest : | maximum density at ca. 3 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in microbiological culture, PCR assays |
| Immunology : | CD2 +, CD3 +, CD4 -, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta +, TCRgamma/delta -; image |
| Fingerprint : | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Cytogenetics : | human diploid karyotype with 2% polyploidy; 46(44-46)<2n>XY; no consistent abnormality detected |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |