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MHH-TALL-2
MHH-TALL-2
规格:
货期:
编号:TS260207
品牌:Testobio
产品名称: MHH-TALL-2
商品货号: TS260207
<strong>Cell line </strong>: MHH-TALL-2
DSMZ no. : TS260207
Species : human (Homo sapiens)
Cell type : T cell acute lymphoblastic leukemia
Origin : established in 1994 from the peripheral blood of a boy with T cell acute lymphoblastic leukemia (T-ALL)
Reference(s) : 16747
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: single cells growing in suspension; image; image
Medium : 80% RPMI 1640 + 20% h.i. FBS
Subculture : seed out at ca. 1-1.5 x 106 cells/ml in 24-well plates; maintain at more than 1 x 106 cells/ml; split saturated culture 1:2 every 3 days; cells are difficult to culture; possibly no cell growth during the first week after thawing
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 60-72 hours
Harvest : maximum density at ca. 3 x 106 cells/ml
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in microbiological culture, PCR assays
Immunology : CD2 +, CD3 +, CD4 -, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta +, TCRgamma/delta -; image
Fingerprint : fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile
Cytogenetics : human diploid karyotype with 2% polyploidy; 46(44-46)<2n>XY; no consistent abnormality detected
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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MHH-TALL-2

  • 货号: TS260207
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  • 品牌 : TESTOBIO
产品名称: MHH-TALL-2
商品货号: TS260207
<strong>Cell line </strong>: MHH-TALL-2
DSMZ no. : TS260207
Species : human (Homo sapiens)
Cell type : T cell acute lymphoblastic leukemia
Origin : established in 1994 from the peripheral blood of a boy with T cell acute lymphoblastic leukemia (T-ALL)
Reference(s) : 16747
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: single cells growing in suspension; image; image
Medium : 80% RPMI 1640 + 20% h.i. FBS
Subculture : seed out at ca. 1-1.5 x 106 cells/ml in 24-well plates; maintain at more than 1 x 106 cells/ml; split saturated culture 1:2 every 3 days; cells are difficult to culture; possibly no cell growth during the first week after thawing
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 60-72 hours
Harvest : maximum density at ca. 3 x 106 cells/ml
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in microbiological culture, PCR assays
Immunology : CD2 +, CD3 +, CD4 -, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta +, TCRgamma/delta -; image
Fingerprint : fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile
Cytogenetics : human diploid karyotype with 2% polyploidy; 46(44-46)<2n>XY; no consistent abnormality detected
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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