T-47D
T-47D
规格:
货期:
编号:TS260219
品牌:Testobio
| 产品名称： | T-47D |
|---|---|
| 商品货号： | TS260219 |
| <strong>Cell line </strong>： | T-47D |
| DSMZ no. ： | TS260219 |
| Species ： | human (Homo sapiens) |
| Cell type ： | breast carcinoma |
| Origin ： | established from the pleural effusion of a 54-year-old woman with infiltrating ductal carcinoma of the breast in 1974; cells were described in the literature to highly express the Wnt-7b oncogene, the progesterone receptor and low amounts of androgene, glucocorticoid and estradiol receptors |
| Reference(s) ： | 17267, 17268, 17269 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells growing adherently as monolayer; image; image |
| Medium ： | 85% RPMI 1640 + 15% h.i. FBS + 10 µg/ml human insulin |
| Subculture ： | seed out at ca. 2-4 x 106 cells/175 cm2 flask; split confluent culture 1:3 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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周雪
13372109301
| 产品名称： | T-47D |
|---|---|
| 商品货号： | TS260219 |
| <strong>Cell line </strong>： | T-47D |
| DSMZ no. ： | TS260219 |
| Species ： | human (Homo sapiens) |
| Cell type ： | breast carcinoma |
| Origin ： | established from the pleural effusion of a 54-year-old woman with infiltrating ductal carcinoma of the breast in 1974; cells were described in the literature to highly express the Wnt-7b oncogene, the progesterone receptor and low amounts of androgene, glucocorticoid and estradiol receptors |
| Reference(s) ： | 17267, 17268, 17269 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells growing adherently as monolayer; image; image |
| Medium ： | 85% RPMI 1640 + 15% h.i. FBS + 10 µg/ml human insulin |
| Subculture ： | seed out at ca. 2-4 x 106 cells/175 cm2 flask; split confluent culture 1:3 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
