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BALL-1
BALL-1
规格:
货期:
编号:TS260220
品牌:Testobio
产品名称: BALL-1
商品货号: TS260220
<strong>Cell line </strong>: BALL-1
DSMZ no. : TS260220
Species : human (Homo sapiens)
Cell type : B cell leukemia
Origin : established in 1976 from the peripheral blood of a 75-year-old man with acute lymphoblastic leukemia (ALL) at diagnosis
Reference(s) : 14772, 19374
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: single round cells growing in suspension; image; image ; image
Medium : 90% RPMI 1640 + 10% h.i. FBS
Subculture : seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-2 x 106 cells/ml; optimal split ratio 1:2 - 1:5 every 2-5 days
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 48-72 hours
Harvest : maximum density at ca. 3 x 106 cells/ml
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : CD3 -, CD5 +, CD8 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, HLA-DR +, sm/cyIgG -, sm/cy IgM +, sm/cykappa -, sm/cylambda +; image
Fingerprint : genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database
Cytogenetics : complex human near-pseudodiploid karyotype; 46(43-47)<2n>XY, der(1)t(1;3)(q32;q25) t(1;6)(p34;p21), der(3)t(1;3)(q32;q25)t(3;15)(p21;q21), der(4)t(4;18)(p11;q21)t(18;1)(q23;q21), der(6)del(6)(q23)t(2;6)(q31;p21), inv(6)(p22q25), t(8;14)(q24;q32)x1-2, del(9)(p22p23)der(9)t(3;9)(q11;p13), t(11;14)(q13;q32), der(11)t(8;11)(q22;q24), del(13)(q13q14), dup(13)(q21q31), der(14)t(14;15)(q32;q21), der(15)t(3;15)(q27;q21), der(15)t(8;15)(q24;q21), der(17)t(8;17)(q24;p11), der(18)qdp(18)(q12q21)t(4;18)(q22;p14), dup(19)(p11p13); sdl with der(14)t(11;14)x2; carries biallelic rearrangements of IGH with CCND1 and MYC, respectively, together with focal amplification of the BCL2/18q21 region which remains unrearranged; resembles published karyotype
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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BALL-1

  • 货号: TS260220
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: BALL-1
商品货号: TS260220
<strong>Cell line </strong>: BALL-1
DSMZ no. : TS260220
Species : human (Homo sapiens)
Cell type : B cell leukemia
Origin : established in 1976 from the peripheral blood of a 75-year-old man with acute lymphoblastic leukemia (ALL) at diagnosis
Reference(s) : 14772, 19374
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: single round cells growing in suspension; image; image ; image
Medium : 90% RPMI 1640 + 10% h.i. FBS
Subculture : seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-2 x 106 cells/ml; optimal split ratio 1:2 - 1:5 every 2-5 days
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 48-72 hours
Harvest : maximum density at ca. 3 x 106 cells/ml
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : CD3 -, CD5 +, CD8 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, HLA-DR +, sm/cyIgG -, sm/cy IgM +, sm/cykappa -, sm/cylambda +; image
Fingerprint : genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database
Cytogenetics : complex human near-pseudodiploid karyotype; 46(43-47)<2n>XY, der(1)t(1;3)(q32;q25) t(1;6)(p34;p21), der(3)t(1;3)(q32;q25)t(3;15)(p21;q21), der(4)t(4;18)(p11;q21)t(18;1)(q23;q21), der(6)del(6)(q23)t(2;6)(q31;p21), inv(6)(p22q25), t(8;14)(q24;q32)x1-2, del(9)(p22p23)der(9)t(3;9)(q11;p13), t(11;14)(q13;q32), der(11)t(8;11)(q22;q24), del(13)(q13q14), dup(13)(q21q31), der(14)t(14;15)(q32;q21), der(15)t(3;15)(q27;q21), der(15)t(8;15)(q24;q21), der(17)t(8;17)(q24;p11), der(18)qdp(18)(q12q21)t(4;18)(q22;p14), dup(19)(p11p13); sdl with der(14)t(11;14)x2; carries biallelic rearrangements of IGH with CCND1 and MYC, respectively, together with focal amplification of the BCL2/18q21 region which remains unrearranged; resembles published karyotype
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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