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HD-MB03
HD-MB03
规格:
货期:
编号:TS260222
品牌:Testobio
产品名称: HD-MB03
商品货号: TS260222
<strong>Cell line </strong>: HD-MB03
DSMZ no. : TS260222
Species : human (Homo sapiens)
Cell type : medulloblastoma
Origin : established in 2011 from a metastasized group 3 medulloblastoma in the cerebellum of a 3-year-old boy; cells were described in the literature to be highly sensitive to histone deacetylase inhibitors
Reference(s) : 17350
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: epithelioid cells growing adherently in monolayers; image; image
Medium : 90% RPMI 1640 + 10% h.i. FBS
Subculture : seed out at ca. 2-3 x 106 cells/80 cm2 flask; split confluent culture 1:2 - 1:3 twice a week using trypsin/EDTA
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 48 hours
Harvest : cell harvest of ca. 10 x 106 cells/80 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint : genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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HD-MB03

  • 货号: TS260222
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  • 品牌 : TESTOBIO
产品名称: HD-MB03
商品货号: TS260222
<strong>Cell line </strong>: HD-MB03
DSMZ no. : TS260222
Species : human (Homo sapiens)
Cell type : medulloblastoma
Origin : established in 2011 from a metastasized group 3 medulloblastoma in the cerebellum of a 3-year-old boy; cells were described in the literature to be highly sensitive to histone deacetylase inhibitors
Reference(s) : 17350
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: epithelioid cells growing adherently in monolayers; image; image
Medium : 90% RPMI 1640 + 10% h.i. FBS
Subculture : seed out at ca. 2-3 x 106 cells/80 cm2 flask; split confluent culture 1:2 - 1:3 twice a week using trypsin/EDTA
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 48 hours
Harvest : cell harvest of ca. 10 x 106 cells/80 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint : genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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