| 产品名称: | JOPACA-1 |
|---|---|
| 商品货号: | TS260234 |
| <strong>Cell line </strong>: | JOPACA-1 |
| DSMZ no. : | TS260234 |
| Species : | human (Homo sapiens) |
| Cell type : | pancreas carcinoma |
| Origin : | established in 2010 from the pancreatic tumor of a 46-year old man with poorly differentiated ductal adenocarcinoma (high grade G3, pT3pN1) |
| Reference(s) : | 17745 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | polymorphic cells growing adherently in monolayers; image; image |
| Medium : | 80% Iscoves MDM + 20% h.i. FBS |
| Subculture : | seed out at ca. 2 x 106 cells/80 cm2 flask; split confluent culture 1:3 to 1:4 once a week using trypsin/EDTA for up to 20 min. |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 3-4 days |
| Harvest : | cell harvest of ca. 10 x 106 cells/175 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| 产品名称: | JOPACA-1 |
|---|---|
| 商品货号: | TS260234 |
| <strong>Cell line </strong>: | JOPACA-1 |
| DSMZ no. : | TS260234 |
| Species : | human (Homo sapiens) |
| Cell type : | pancreas carcinoma |
| Origin : | established in 2010 from the pancreatic tumor of a 46-year old man with poorly differentiated ductal adenocarcinoma (high grade G3, pT3pN1) |
| Reference(s) : | 17745 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | polymorphic cells growing adherently in monolayers; image; image |
| Medium : | 80% Iscoves MDM + 20% h.i. FBS |
| Subculture : | seed out at ca. 2 x 106 cells/80 cm2 flask; split confluent culture 1:3 to 1:4 once a week using trypsin/EDTA for up to 20 min. |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 3-4 days |
| Harvest : | cell harvest of ca. 10 x 106 cells/175 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |