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JOPACA-1
JOPACA-1
规格:
货期:
编号:TS260234
品牌:Testobio
产品名称: JOPACA-1
商品货号: TS260234
<strong>Cell line </strong>: JOPACA-1
DSMZ no. : TS260234
Species : human (Homo sapiens)
Cell type : pancreas carcinoma
Origin : established in 2010 from the pancreatic tumor of a 46-year old man with poorly differentiated ductal adenocarcinoma (high grade G3, pT3pN1)
Reference(s) : 17745
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: polymorphic cells growing adherently in monolayers; image; image
Medium : 80% Iscoves MDM + 20% h.i. FBS
Subculture : seed out at ca. 2 x 106 cells/80 cm2 flask; split confluent culture 1:3 to 1:4 once a week using trypsin/EDTA for up to 20 min.
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 3-4 days
Harvest : cell harvest of ca. 10 x 106 cells/175 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint : genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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JOPACA-1

  • 货号: TS260234
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  • 品牌 : TESTOBIO
产品名称: JOPACA-1
商品货号: TS260234
<strong>Cell line </strong>: JOPACA-1
DSMZ no. : TS260234
Species : human (Homo sapiens)
Cell type : pancreas carcinoma
Origin : established in 2010 from the pancreatic tumor of a 46-year old man with poorly differentiated ductal adenocarcinoma (high grade G3, pT3pN1)
Reference(s) : 17745
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: polymorphic cells growing adherently in monolayers; image; image
Medium : 80% Iscoves MDM + 20% h.i. FBS
Subculture : seed out at ca. 2 x 106 cells/80 cm2 flask; split confluent culture 1:3 to 1:4 once a week using trypsin/EDTA for up to 20 min.
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 3-4 days
Harvest : cell harvest of ca. 10 x 106 cells/175 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint : genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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