GCT-27
GCT-27
规格:
货期:
编号:TS260236
品牌:Testobio
| 产品名称： | GCT-27 |
|---|---|
| 商品货号： | TS260236 |
| <strong>Cell line </strong>： | GCT-27 |
| DSMZ no. ： | TS260236 |
| Species ： | human (Homo sapiens) |
| Cell type ： | testicular germ cell tumor |
| Origin ： | established from the germ cell tumor of a man with a malignant testicular teratoma (intermediate grade) |
| Reference(s) ： | 17682, 15192 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in monolayers in collagen-coated flasks; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 100 nM hydrocortisone |
| Subculture ： | seed out at ca. 1-1.5 x 106 cells/175 cm2 collagen-coated flask; split confluent culture 1:7 to 1:8 every 4-6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35-40 hours |
| Harvest ： | cell harvest of ca. 7-8 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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| 产品名称： | GCT-27 |
|---|---|
| 商品货号： | TS260236 |
| <strong>Cell line </strong>： | GCT-27 |
| DSMZ no. ： | TS260236 |
| Species ： | human (Homo sapiens) |
| Cell type ： | testicular germ cell tumor |
| Origin ： | established from the germ cell tumor of a man with a malignant testicular teratoma (intermediate grade) |
| Reference(s) ： | 17682, 15192 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in monolayers in collagen-coated flasks; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 100 nM hydrocortisone |
| Subculture ： | seed out at ca. 1-1.5 x 106 cells/175 cm2 collagen-coated flask; split confluent culture 1:7 to 1:8 every 4-6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35-40 hours |
| Harvest ： | cell harvest of ca. 7-8 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
