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U-CH2
U-CH2
规格:
货期:
编号:TS260239
品牌:Testobio
产品名称: U-CH2
商品货号: TS260239
<strong>Cell line </strong>: U-CH2
DSMZ no. : TS260239
Species : human (Homo sapiens)
Cell type : chordoma
Origin : established in 2000 from the malignant sacral chordoma of a 72-year-old Caucasian woman; cell line is described in the literature having a complex karyotype with dmins and manifold chromosomal abberrations
Reference(s) : 17941
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: typical physalipherous chordoma phenotype growing strictly adherent ; image
Medium : 80% mixture of Iscoves MDM and RPMI 1640 (at 4:1) + 20% FBS (do not heat-inactivate!) + 2 mM glutamine
Subculture : seed out at ca. 0.5 x 106 cells/80 cm2 flask; split confluent culture every 6-8 days using trypsin/EDTA or TrypLE Express
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 5-7 days
Harvest : cell harvest of ca. 3 x 106 cells/80 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint : genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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U-CH2

  • 货号: TS260239
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  • 品牌 : TESTOBIO
产品名称: U-CH2
商品货号: TS260239
<strong>Cell line </strong>: U-CH2
DSMZ no. : TS260239
Species : human (Homo sapiens)
Cell type : chordoma
Origin : established in 2000 from the malignant sacral chordoma of a 72-year-old Caucasian woman; cell line is described in the literature having a complex karyotype with dmins and manifold chromosomal abberrations
Reference(s) : 17941
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: typical physalipherous chordoma phenotype growing strictly adherent ; image
Medium : 80% mixture of Iscoves MDM and RPMI 1640 (at 4:1) + 20% FBS (do not heat-inactivate!) + 2 mM glutamine
Subculture : seed out at ca. 0.5 x 106 cells/80 cm2 flask; split confluent culture every 6-8 days using trypsin/EDTA or TrypLE Express
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 5-7 days
Harvest : cell harvest of ca. 3 x 106 cells/80 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint : genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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