| 产品名称: | U-CH2 |
|---|---|
| 商品货号: | TS260239 |
| <strong>Cell line </strong>: | U-CH2 |
| DSMZ no. : | TS260239 |
| Species : | human (Homo sapiens) |
| Cell type : | chordoma |
| Origin : | established in 2000 from the malignant sacral chordoma of a 72-year-old Caucasian woman; cell line is described in the literature having a complex karyotype with dmins and manifold chromosomal abberrations |
| Reference(s) : | 17941 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | typical physalipherous chordoma phenotype growing strictly adherent ; image |
| Medium : | 80% mixture of Iscoves MDM and RPMI 1640 (at 4:1) + 20% FBS (do not heat-inactivate!) + 2 mM glutamine |
| Subculture : | seed out at ca. 0.5 x 106 cells/80 cm2 flask; split confluent culture every 6-8 days using trypsin/EDTA or TrypLE Express |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 5-7 days |
| Harvest : | cell harvest of ca. 3 x 106 cells/80 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| 产品名称: | U-CH2 |
|---|---|
| 商品货号: | TS260239 |
| <strong>Cell line </strong>: | U-CH2 |
| DSMZ no. : | TS260239 |
| Species : | human (Homo sapiens) |
| Cell type : | chordoma |
| Origin : | established in 2000 from the malignant sacral chordoma of a 72-year-old Caucasian woman; cell line is described in the literature having a complex karyotype with dmins and manifold chromosomal abberrations |
| Reference(s) : | 17941 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | typical physalipherous chordoma phenotype growing strictly adherent ; image |
| Medium : | 80% mixture of Iscoves MDM and RPMI 1640 (at 4:1) + 20% FBS (do not heat-inactivate!) + 2 mM glutamine |
| Subculture : | seed out at ca. 0.5 x 106 cells/80 cm2 flask; split confluent culture every 6-8 days using trypsin/EDTA or TrypLE Express |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 5-7 days |
| Harvest : | cell harvest of ca. 3 x 106 cells/80 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |