| 产品名称: | SUSA |
|---|---|
| 商品货号: | TS260243 |
| <strong>Cell line </strong>: | SUSA |
| DSMZ no. : | TS260243 |
| Species : | human (Homo sapiens) |
| Cell type : | testicular germ cell tumor |
| Origin : | established in 1977 from a 30-year old man with a malignant testicular teratoma (intermediate grade B) with extensive necrosis |
| Reference(s) : | 17589 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelioid cells growing adherently in monolayers; image |
| Medium : | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture : | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 - 1:4 every 2-4 days using trypsin/EDTA |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 48-72 hours |
| Harvest : | cell harvest of ca. 5 x 106 cells/80 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| 产品名称: | SUSA |
|---|---|
| 商品货号: | TS260243 |
| <strong>Cell line </strong>: | SUSA |
| DSMZ no. : | TS260243 |
| Species : | human (Homo sapiens) |
| Cell type : | testicular germ cell tumor |
| Origin : | established in 1977 from a 30-year old man with a malignant testicular teratoma (intermediate grade B) with extensive necrosis |
| Reference(s) : | 17589 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelioid cells growing adherently in monolayers; image |
| Medium : | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture : | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 - 1:4 every 2-4 days using trypsin/EDTA |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 48-72 hours |
| Harvest : | cell harvest of ca. 5 x 106 cells/80 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |