U-CH1
U-CH1
规格:
货期:
编号:TS260244
品牌:Testobio
| 产品名称： | U-CH1 |
|---|---|
| 商品货号： | TS260244 |
| <strong>Cell line </strong>： | U-CH1 |
| DSMZ no. ： | TS260244 |
| Species ： | human (Homo sapiens) |
| Cell type ： | chordoma |
| Origin ： | established in 1998 from the local recurrence of a malignant sacral chordoma in a 46-year-old Caucasian man four years after radiotherapy |
| Reference(s) ： | 17940, 17941 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | semi-adherent, granular collagen-rich cells of irregular shape; image; image ; image |
| Medium ： | 80% mixture of Iscoves MDM and RPMI 1640 (at 4:1) + 20% FBS (do not heat-inactivate!) |
| Subculture ： | seed out at ca. 7 x 106 cells/80 cm2 flask; split confluent culture 1:2; use trypsin/EDTA for subculture, although cells detach easily without trypsin/EDTA; trypsinized cells adhere evenly to the bottom of the vessel, whereas non-trypsinized cells grow as clumps in suspension; as the cells are quite loosely attached, the culture vessels should be handled cautiously when spent medium is exchanged |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 1 week (may be significantly longer after LN(2) recovery) |
| Harvest ： | not definable |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
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李晶晶
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周雪
13372109301
| 产品名称： | U-CH1 |
|---|---|
| 商品货号： | TS260244 |
| <strong>Cell line </strong>： | U-CH1 |
| DSMZ no. ： | TS260244 |
| Species ： | human (Homo sapiens) |
| Cell type ： | chordoma |
| Origin ： | established in 1998 from the local recurrence of a malignant sacral chordoma in a 46-year-old Caucasian man four years after radiotherapy |
| Reference(s) ： | 17940, 17941 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | semi-adherent, granular collagen-rich cells of irregular shape; image; image ; image |
| Medium ： | 80% mixture of Iscoves MDM and RPMI 1640 (at 4:1) + 20% FBS (do not heat-inactivate!) |
| Subculture ： | seed out at ca. 7 x 106 cells/80 cm2 flask; split confluent culture 1:2; use trypsin/EDTA for subculture, although cells detach easily without trypsin/EDTA; trypsinized cells adhere evenly to the bottom of the vessel, whereas non-trypsinized cells grow as clumps in suspension; as the cells are quite loosely attached, the culture vessels should be handled cautiously when spent medium is exchanged |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 1 week (may be significantly longer after LN(2) recovery) |
| Harvest ： | not definable |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
