| 产品名称: | MUG-CHOR-1 |
|---|---|
| 商品货号: | TS260252 |
| <strong>Cell line </strong>: | MUG-CHOR-1 |
| DSMZ no. : | TS260252 |
| Species : | human (Homo sapiens) |
| Cell type : | chordoma |
| Origin : | established in 2006 from a recurrent morphologically "classic" sacrococcygeal chordoma of a 58-year-old Caucasian woman |
| Reference(s) : | 18729 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelioid cells growing adherently in monolayers; image |
| Medium : | 80% mixture of Iscoves MDM and RPMI 1640 (at 5:1) + 20% h.i. FBS + 1% insulin-transferrin-sodium selenite (IST) + 2 mM L-glutamine |
| Subculture : | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:3 once or twice a week using trypsin/EDTA |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 75-100 hours |
| Harvest : | cell harvest of ca. 4 x 106 cells/80 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| 产品名称: | MUG-CHOR-1 |
|---|---|
| 商品货号: | TS260252 |
| <strong>Cell line </strong>: | MUG-CHOR-1 |
| DSMZ no. : | TS260252 |
| Species : | human (Homo sapiens) |
| Cell type : | chordoma |
| Origin : | established in 2006 from a recurrent morphologically "classic" sacrococcygeal chordoma of a 58-year-old Caucasian woman |
| Reference(s) : | 18729 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelioid cells growing adherently in monolayers; image |
| Medium : | 80% mixture of Iscoves MDM and RPMI 1640 (at 5:1) + 20% h.i. FBS + 1% insulin-transferrin-sodium selenite (IST) + 2 mM L-glutamine |
| Subculture : | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:3 once or twice a week using trypsin/EDTA |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 75-100 hours |
| Harvest : | cell harvest of ca. 4 x 106 cells/80 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |