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MUG-CHOR-1
MUG-CHOR-1
规格:
货期:
编号:TS260252
品牌:Testobio
产品名称: MUG-CHOR-1
商品货号: TS260252
<strong>Cell line </strong>: MUG-CHOR-1
DSMZ no. : TS260252
Species : human (Homo sapiens)
Cell type : chordoma
Origin : established in 2006 from a recurrent morphologically "classic" sacrococcygeal chordoma of a 58-year-old Caucasian woman
Reference(s) : 18729
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: epithelioid cells growing adherently in monolayers; image
Medium : 80% mixture of Iscoves MDM and RPMI 1640 (at 5:1) + 20% h.i. FBS + 1% insulin-transferrin-sodium selenite (IST) + 2 mM L-glutamine
Subculture : seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:3 once or twice a week using trypsin/EDTA
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 75-100 hours
Harvest : cell harvest of ca. 4 x 106 cells/80 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint : genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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MUG-CHOR-1

  • 货号: TS260252
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  • 品牌 : TESTOBIO
产品名称: MUG-CHOR-1
商品货号: TS260252
<strong>Cell line </strong>: MUG-CHOR-1
DSMZ no. : TS260252
Species : human (Homo sapiens)
Cell type : chordoma
Origin : established in 2006 from a recurrent morphologically "classic" sacrococcygeal chordoma of a 58-year-old Caucasian woman
Reference(s) : 18729
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: epithelioid cells growing adherently in monolayers; image
Medium : 80% mixture of Iscoves MDM and RPMI 1640 (at 5:1) + 20% h.i. FBS + 1% insulin-transferrin-sodium selenite (IST) + 2 mM L-glutamine
Subculture : seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:3 once or twice a week using trypsin/EDTA
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 75-100 hours
Harvest : cell harvest of ca. 4 x 106 cells/80 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint : genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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