| 产品名称: | BXPC-3 |
|---|---|
| 商品货号: | TS260260 |
| <strong>Cell line </strong>: | BXPC-3 |
| DSMZ no. : | TS260260 |
| Species : | human (Homo sapiens) |
| Cell type : | pancreas carcinoma |
| Origin : | established in 1980 from the biopsy specimen of the primary adenocarcinoma of the body of the pancreas from a 61-year-old woman; cells were described not to express cystic fibrosis transmembrane conductance regulator (CFTR) |
| Reference(s) : | 18730, 18731 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelial-like cells growing adherently as monolayer in tight clusters or islands; single cells sometimes with long extensions; image; image; image |
| Medium : | 80-90% RPMI 1640 + 10-20% h.i. FBS + 2 mM L-glutamine |
| Subculture : | seed out at ca. 2 x 106 cells/80 cm2 flask; split confluent culture 1:2 - 1:4 every second day using trypsin/EDTA |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 48 hours |
| Harvest : | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| 产品名称: | BXPC-3 |
|---|---|
| 商品货号: | TS260260 |
| <strong>Cell line </strong>: | BXPC-3 |
| DSMZ no. : | TS260260 |
| Species : | human (Homo sapiens) |
| Cell type : | pancreas carcinoma |
| Origin : | established in 1980 from the biopsy specimen of the primary adenocarcinoma of the body of the pancreas from a 61-year-old woman; cells were described not to express cystic fibrosis transmembrane conductance regulator (CFTR) |
| Reference(s) : | 18730, 18731 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelial-like cells growing adherently as monolayer in tight clusters or islands; single cells sometimes with long extensions; image; image; image |
| Medium : | 80-90% RPMI 1640 + 10-20% h.i. FBS + 2 mM L-glutamine |
| Subculture : | seed out at ca. 2 x 106 cells/80 cm2 flask; split confluent culture 1:2 - 1:4 every second day using trypsin/EDTA |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 48 hours |
| Harvest : | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |