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BXPC-3
BXPC-3
规格:
货期:
编号:TS260260
品牌:Testobio
产品名称: BXPC-3
商品货号: TS260260
<strong>Cell line </strong>: BXPC-3
DSMZ no. : TS260260
Species : human (Homo sapiens)
Cell type : pancreas carcinoma
Origin : established in 1980 from the biopsy specimen of the primary adenocarcinoma of the body of the pancreas from a 61-year-old woman; cells were described not to express cystic fibrosis transmembrane conductance regulator (CFTR)
Reference(s) : 18730, 18731
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: epithelial-like cells growing adherently as monolayer in tight clusters or islands; single cells sometimes with long extensions; image; image; image
Medium : 80-90% RPMI 1640 + 10-20% h.i. FBS + 2 mM L-glutamine
Subculture : seed out at ca. 2 x 106 cells/80 cm2 flask; split confluent culture 1:2 - 1:4 every second day using trypsin/EDTA
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 48 hours
Harvest : cell harvest of ca. 10 x 106 cells/80 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint : genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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BXPC-3

  • 货号: TS260260
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  • 品牌 : TESTOBIO
产品名称: BXPC-3
商品货号: TS260260
<strong>Cell line </strong>: BXPC-3
DSMZ no. : TS260260
Species : human (Homo sapiens)
Cell type : pancreas carcinoma
Origin : established in 1980 from the biopsy specimen of the primary adenocarcinoma of the body of the pancreas from a 61-year-old woman; cells were described not to express cystic fibrosis transmembrane conductance regulator (CFTR)
Reference(s) : 18730, 18731
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: epithelial-like cells growing adherently as monolayer in tight clusters or islands; single cells sometimes with long extensions; image; image; image
Medium : 80-90% RPMI 1640 + 10-20% h.i. FBS + 2 mM L-glutamine
Subculture : seed out at ca. 2 x 106 cells/80 cm2 flask; split confluent culture 1:2 - 1:4 every second day using trypsin/EDTA
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 48 hours
Harvest : cell harvest of ca. 10 x 106 cells/80 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint : genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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