BJAB
BJAB
规格:
货期:
编号:TS260262
品牌:Testobio
| 产品名称： | BJAB |
|---|---|
| 商品货号： | TS260262 |
| <strong>Cell line </strong>： | BJAB |
| DSMZ no. ： | TS260262 |
| Species ： | human |
| Cell type ： | Burkitt lymphoma |
| Origin ： | established in 1973 from the inguinal tumor of an EBV-negative 5-year-old African girl with Burkitt lymphoma at the preterminal stage; although the tumor tissue was experimentally exposed to EBV, neither EBV sequences nor EBV antigens were detectable in the established cell line. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 18899, 15230, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorphic cells growing in suspension singly cells and in clumps; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-0.7 x 106 cells/ml; optimal split ratio 1:2 -1:5 every 2-3 days; culture flask should be kept horizontal |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Cytogenetics ： | human hypodiploid karyotype; 44(40-46)<2n>XX, -10, -21, der(6)t(6;10)(p22;p12), der(7)t(1;7)(p36;p22), t(11;17)(q24;q24), der(11)t(11;17)(q24;q24)t(11;6)(p15;q11)t(6;5)(q22;?), ider(13)(q10)t(3;13)(q26;q14), der(13)t(10;13)(q11;p11)dup(13)(q11q32)t(13;21)(q32;q22), der(21)t(13;21); does not correspond with previous BJA-B karyotypes published; no IG/MYC translocation detected |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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testobio@163.com
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | BJAB |
|---|---|
| 商品货号： | TS260262 |
| <strong>Cell line </strong>： | BJAB |
| DSMZ no. ： | TS260262 |
| Species ： | human |
| Cell type ： | Burkitt lymphoma |
| Origin ： | established in 1973 from the inguinal tumor of an EBV-negative 5-year-old African girl with Burkitt lymphoma at the preterminal stage; although the tumor tissue was experimentally exposed to EBV, neither EBV sequences nor EBV antigens were detectable in the established cell line. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 18899, 15230, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to polymorphic cells growing in suspension singly cells and in clumps; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-0.7 x 106 cells/ml; optimal split ratio 1:2 -1:5 every 2-3 days; culture flask should be kept horizontal |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | maximum density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Cytogenetics ： | human hypodiploid karyotype; 44(40-46)<2n>XX, -10, -21, der(6)t(6;10)(p22;p12), der(7)t(1;7)(p36;p22), t(11;17)(q24;q24), der(11)t(11;17)(q24;q24)t(11;6)(p15;q11)t(6;5)(q22;?), ider(13)(q10)t(3;13)(q26;q14), der(13)t(10;13)(q11;p11)dup(13)(q11q32)t(13;21)(q32;q22), der(21)t(13;21); does not correspond with previous BJA-B karyotypes published; no IG/MYC translocation detected |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
