LS-174T
LS-174T
规格:
货期:
编号:TS260266
品牌:Testobio
| 产品名称： | LS-174T |
|---|---|
| 商品货号： | TS260266 |
| <strong>Cell line </strong>： | LS-174T |
| DSMZ no. ： | TS260266 |
| Species ： | human (Homo sapiens) |
| Cell type ： | colon adenocarcinoma |
| Origin ： | established in 1974 from the moderately well-differentiated primary colonic adenocarcinoma (Duke´s type B) of a 58-year-old woman; cells were described in the literature to produce large amounts of carcinoembryonic antigen (CEA) and to express C-Myc, N-Myc, H-Ras, and N-Ras |
| Reference(s) ： | 18716 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing adherently as monolayer in tight clusters or islands; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 2-4 x 106 cells/80 cm2 flask; split confluent culture 1:5 - 1:10 every 3-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 20-25 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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周雪
13372109301
| 产品名称： | LS-174T |
|---|---|
| 商品货号： | TS260266 |
| <strong>Cell line </strong>： | LS-174T |
| DSMZ no. ： | TS260266 |
| Species ： | human (Homo sapiens) |
| Cell type ： | colon adenocarcinoma |
| Origin ： | established in 1974 from the moderately well-differentiated primary colonic adenocarcinoma (Duke´s type B) of a 58-year-old woman; cells were described in the literature to produce large amounts of carcinoembryonic antigen (CEA) and to express C-Myc, N-Myc, H-Ras, and N-Ras |
| Reference(s) ： | 18716 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing adherently as monolayer in tight clusters or islands; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 2-4 x 106 cells/80 cm2 flask; split confluent culture 1:5 - 1:10 every 3-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 20-25 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
