CI
CI
规格:
货期:
编号:TS260297
品牌:Testobio
| 产品名称： | CI |
|---|---|
| 商品货号： | TS260297 |
| <strong>Cell line </strong>： | CI |
| DSMZ no. ： | TS260297 |
| Species ： | human (Homo sapiens) |
| Cell type ： | chronic B cell leukemia |
| Origin ： | established in 1978 from Epstein-Barr virus (EBV) immortalized lymphocytes of a 47-year-old woman with chronic lymphocytic leukemia (CLL, Rai II stage at diagnosis); cell line - formerly thought of as a B-lymphoblastoid sister - is now deemed a subclone of the putatively oligoclonal CII cell line; cells express stereotyped subset 5 BCR |
| Reference(s) ： | 19602, 19603, 19604, 19172 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small spheroidal or ovoid cells bearing occasional processes, growing singly or in clumps; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1 x 106 cells/ml; optimal split ratio 1:2 - 1:4 every 2-4 days; growth of cells is feeder-layer independent |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-60 hours |
| Harvest ： | maximum density at ca. 4 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD5 +, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR revealed authentic STR DNA profile identical to CII |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype with 9% polyploidy; 46-50<2n>XX, +12, del(13)(q14q22); sdl with +3 without del(13); +12 and del(13) recurrent in CLL; resembles published karyotype |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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周雪
13372109301
| 产品名称： | CI |
|---|---|
| 商品货号： | TS260297 |
| <strong>Cell line </strong>： | CI |
| DSMZ no. ： | TS260297 |
| Species ： | human (Homo sapiens) |
| Cell type ： | chronic B cell leukemia |
| Origin ： | established in 1978 from Epstein-Barr virus (EBV) immortalized lymphocytes of a 47-year-old woman with chronic lymphocytic leukemia (CLL, Rai II stage at diagnosis); cell line - formerly thought of as a B-lymphoblastoid sister - is now deemed a subclone of the putatively oligoclonal CII cell line; cells express stereotyped subset 5 BCR |
| Reference(s) ： | 19602, 19603, 19604, 19172 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small spheroidal or ovoid cells bearing occasional processes, growing singly or in clumps; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1 x 106 cells/ml; optimal split ratio 1:2 - 1:4 every 2-4 days; growth of cells is feeder-layer independent |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-60 hours |
| Harvest ： | maximum density at ca. 4 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD3 -, CD5 +, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | fluorescent nonaplex PCR revealed authentic STR DNA profile identical to CII |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype with 9% polyploidy; 46-50<2n>XX, +12, del(13)(q14q22); sdl with +3 without del(13); +12 and del(13) recurrent in CLL; resembles published karyotype |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
