| 产品名称: | WSU-DLCL |
|---|---|
| 商品货号: | TS260303 |
| <strong>Cell line </strong>: | WSU-DLCL |
| DSMZ no. : | TS260303 |
| Species : | human (Homo sapiens) |
| Cell type : | B cell lymphoma |
| Origin : | established in 1988 from the malignant pleural fluid of a 32-year-old woman with diffuse large B cell lymphoma at terminal stage |
| Reference(s) : | 24246 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | round cells growing mostly in clumps in suspension |
| Medium : | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture : | seed out at ca. 0.2-0.3 x 106 cells/ml; optimal split ratio 1:2 - 1:3 every 3-4 days; cells start to proliferate after approximately one week |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 48-72 hours |
| Harvest : | maximum density at ca. 0.9 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | CD3 -, CD13 -, CD19 +, CD20 -, CD34 -, HLA-DR -, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint : | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| 产品名称: | WSU-DLCL |
|---|---|
| 商品货号: | TS260303 |
| <strong>Cell line </strong>: | WSU-DLCL |
| DSMZ no. : | TS260303 |
| Species : | human (Homo sapiens) |
| Cell type : | B cell lymphoma |
| Origin : | established in 1988 from the malignant pleural fluid of a 32-year-old woman with diffuse large B cell lymphoma at terminal stage |
| Reference(s) : | 24246 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | round cells growing mostly in clumps in suspension |
| Medium : | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture : | seed out at ca. 0.2-0.3 x 106 cells/ml; optimal split ratio 1:2 - 1:3 every 3-4 days; cells start to proliferate after approximately one week |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 48-72 hours |
| Harvest : | maximum density at ca. 0.9 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | CD3 -, CD13 -, CD19 +, CD20 -, CD34 -, HLA-DR -, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint : | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |