| 产品名称: | FADU |
|---|---|
| 商品货号: | TS260308 |
| <strong>Cell line </strong>: | FADU |
| DSMZ no. : | TS260308 |
| Species : | confirmed as human by STR-typing |
| Cell type : | pharynx carcinoma |
| Origin : | established in 1968 from a squamous cell carcinoma of the hypopharynx grade II of a 56-year-old Indian man; cells were described in the literature to overexpress bcl-1 and to be tumorigenic in mice |
| Reference(s) : | 24158, 14537, 14529 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelioid cells growing adherently as monolayer |
| Medium : | 90% MEM (with Earles salts) + 10% h.i. FBS |
| Subculture : | seed out at ca. 1.5 x 106 cells/175 cm2 flask; split confluent culture 1:10 - 1:20 once a week using trypsin/EDTA |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 30 hours |
| Harvest : | cell harvest of ca. 20-30 x 106 cells/175 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |
| 产品名称: | FADU |
|---|---|
| 商品货号: | TS260308 |
| <strong>Cell line </strong>: | FADU |
| DSMZ no. : | TS260308 |
| Species : | confirmed as human by STR-typing |
| Cell type : | pharynx carcinoma |
| Origin : | established in 1968 from a squamous cell carcinoma of the hypopharynx grade II of a 56-year-old Indian man; cells were described in the literature to overexpress bcl-1 and to be tumorigenic in mice |
| Reference(s) : | 24158, 14537, 14529 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelioid cells growing adherently as monolayer |
| Medium : | 90% MEM (with Earles salts) + 10% h.i. FBS |
| Subculture : | seed out at ca. 1.5 x 106 cells/175 cm2 flask; split confluent culture 1:10 - 1:20 once a week using trypsin/EDTA |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 30 hours |
| Harvest : | cell harvest of ca. 20-30 x 106 cells/175 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |