| 产品名称: | NALM-33 |
|---|---|
| 商品货号: | TS260315 |
| <strong>Cell line </strong>: | NALM-33 |
| DSMZ no. : | TS260315 |
| Species : | confirmed as human by STR-typing |
| Cell type : | B cell precursor leukemia |
| Origin : | established in 1996 from the peripheral blood of a 72-year-old man with B cell precursor (BCP) acute lymphoblastic leukemia (ALL) at relapse |
| Reference(s) : | 18127 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | single round cells growing in suspension |
| Medium : | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture : | seed out at ca. 1 x 106 cells/ml in 24-well plates, maintain at 1-1.5 x 10(6) cells/ml; optimal split ratio 1:2 - 1:3 every 2-3 days |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 48 hours |
| Harvest : | maximum density at ca. 2.5-3 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint : | genetic typing showed a short tandem repeat (STR) profile identical to sister cell line NALM-34 by a search of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| 产品名称: | NALM-33 |
|---|---|
| 商品货号: | TS260315 |
| <strong>Cell line </strong>: | NALM-33 |
| DSMZ no. : | TS260315 |
| Species : | confirmed as human by STR-typing |
| Cell type : | B cell precursor leukemia |
| Origin : | established in 1996 from the peripheral blood of a 72-year-old man with B cell precursor (BCP) acute lymphoblastic leukemia (ALL) at relapse |
| Reference(s) : | 18127 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | single round cells growing in suspension |
| Medium : | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture : | seed out at ca. 1 x 106 cells/ml in 24-well plates, maintain at 1-1.5 x 10(6) cells/ml; optimal split ratio 1:2 - 1:3 every 2-3 days |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 48 hours |
| Harvest : | maximum density at ca. 2.5-3 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint : | genetic typing showed a short tandem repeat (STR) profile identical to sister cell line NALM-34 by a search of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |