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NALM-33
NALM-33
规格:
货期:
编号:TS260315
品牌:Testobio
产品名称: NALM-33
商品货号: TS260315
<strong>Cell line </strong>: NALM-33
DSMZ no. : TS260315
Species : confirmed as human by STR-typing
Cell type : B cell precursor leukemia
Origin : established in 1996 from the peripheral blood of a 72-year-old man with B cell precursor (BCP) acute lymphoblastic leukemia (ALL) at relapse
Reference(s) : 18127
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: single round cells growing in suspension
Medium : 80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture : seed out at ca. 1 x 106 cells/ml in 24-well plates, maintain at 1-1.5 x 10(6) cells/ml; optimal split ratio 1:2 - 1:3 every 2-3 days
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 48 hours
Harvest : maximum density at ca. 2.5-3 x 106 cells/ml
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image
Fingerprint : genetic typing showed a short tandem repeat (STR) profile identical to sister cell line NALM-34 by a search of the international STR database
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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NALM-33

  • 货号: TS260315
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  • 品牌 : TESTOBIO
产品名称: NALM-33
商品货号: TS260315
<strong>Cell line </strong>: NALM-33
DSMZ no. : TS260315
Species : confirmed as human by STR-typing
Cell type : B cell precursor leukemia
Origin : established in 1996 from the peripheral blood of a 72-year-old man with B cell precursor (BCP) acute lymphoblastic leukemia (ALL) at relapse
Reference(s) : 18127
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: single round cells growing in suspension
Medium : 80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture : seed out at ca. 1 x 106 cells/ml in 24-well plates, maintain at 1-1.5 x 10(6) cells/ml; optimal split ratio 1:2 - 1:3 every 2-3 days
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 48 hours
Harvest : maximum density at ca. 2.5-3 x 106 cells/ml
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image
Fingerprint : genetic typing showed a short tandem repeat (STR) profile identical to sister cell line NALM-34 by a search of the international STR database
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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