HS-578T
HS-578T
规格:
货期:
编号:TS260316
品牌:Testobio
| 产品名称： | HS-578T |
|---|---|
| 商品货号： | TS260316 |
| <strong>Cell line </strong>： | HS-578T |
| DSMZ no. ： | TS260316 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | breast carcinoma |
| Origin ： | established in 1977 from the carcinosarcoma of the breast of a 74-year-old Caucasian woman; the cells were described in the literature to produce casein and to lack estrogen-receptor protein |
| Reference(s) ： | 18119, 18120, 18121 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | irregularely shaped epithelioid cells growing adherently in monolayers |
| Medium ： | 90% Dulbecco´s MEM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 - 1:4 every second day using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
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李晶晶
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周雪
13372109301
| 产品名称： | HS-578T |
|---|---|
| 商品货号： | TS260316 |
| <strong>Cell line </strong>： | HS-578T |
| DSMZ no. ： | TS260316 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | breast carcinoma |
| Origin ： | established in 1977 from the carcinosarcoma of the breast of a 74-year-old Caucasian woman; the cells were described in the literature to produce casein and to lack estrogen-receptor protein |
| Reference(s) ： | 18119, 18120, 18121 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | irregularely shaped epithelioid cells growing adherently in monolayers |
| Medium ： | 90% Dulbecco´s MEM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 - 1:4 every second day using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
