U-2-OS
U-2-OS
规格:
货期:
编号:TS260326
品牌:Testobio
| 产品名称： | U-2-OS |
|---|---|
| 商品货号： | TS260326 |
| <strong>Cell line </strong>： | U-2-OS |
| DSMZ no. ： | TS260326 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | osteosarcoma |
| Origin ： | established in 1964 from a moderately differentiated osteogenic sarcoma of the tibia of a 15-year-old Caucasian girl; cells were described in the literature to secrete osteosarcoma-derived growth factor (ODGF), insulin-like growth factor (IGF-1) receptor, insulin-like growth factor-II/Mannose-6-phosphate (IGF-II/M6P) receptor, and IGF-II; the cells were originally designated 2T |
| Reference(s) ： | 24159, 24160, 24161, 24162 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently as monolayer; image; image |
| Medium ： | 90% McCoys 5A + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/175 cm2 flask; split confluent culture 1:10 - 1:20 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 25-30 hours |
| Harvest ： | cell harvest of ca. 7 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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章丽敏
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朱静
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李晶晶
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周雪
13372109301
| 产品名称： | U-2-OS |
|---|---|
| 商品货号： | TS260326 |
| <strong>Cell line </strong>： | U-2-OS |
| DSMZ no. ： | TS260326 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | osteosarcoma |
| Origin ： | established in 1964 from a moderately differentiated osteogenic sarcoma of the tibia of a 15-year-old Caucasian girl; cells were described in the literature to secrete osteosarcoma-derived growth factor (ODGF), insulin-like growth factor (IGF-1) receptor, insulin-like growth factor-II/Mannose-6-phosphate (IGF-II/M6P) receptor, and IGF-II; the cells were originally designated 2T |
| Reference(s) ： | 24159, 24160, 24161, 24162 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently as monolayer; image; image |
| Medium ： | 90% McCoys 5A + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/175 cm2 flask; split confluent culture 1:10 - 1:20 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 25-30 hours |
| Harvest ： | cell harvest of ca. 7 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
