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ETCC-016
ETCC-016
规格:
货期:
编号:TS260342
品牌:Testobio
产品名称: ETCC-016
商品货号: TS260342
<strong>Cell line </strong>: ETCC-016
DSMZ no. : TS260342
Species : confirmed as human by STR-typing
Cell type : lung adenocarcinoma
Origin : established in 2011 from the pleural effusion of a 53-year-old man of Singapore Chinese origin with advanced adenocarcinoma of the lung
Reference(s) : 18126
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: epithelioid cells growing in monolayers ; image
Medium : 90% Dulbecco´s MEM + 10% h.i. FBS
Subculture : seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:3 - 1:4 every second day using trypsin/EDTA
Incubation : at 37 °C with 10% CO2
Doubling time : ca. 50 hours
Harvest : cell harvest of ca. 10-15 x 106 cells/175 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint : genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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ETCC-016

  • 货号: TS260342
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: ETCC-016
商品货号: TS260342
<strong>Cell line </strong>: ETCC-016
DSMZ no. : TS260342
Species : confirmed as human by STR-typing
Cell type : lung adenocarcinoma
Origin : established in 2011 from the pleural effusion of a 53-year-old man of Singapore Chinese origin with advanced adenocarcinoma of the lung
Reference(s) : 18126
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: epithelioid cells growing in monolayers ; image
Medium : 90% Dulbecco´s MEM + 10% h.i. FBS
Subculture : seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:3 - 1:4 every second day using trypsin/EDTA
Incubation : at 37 °C with 10% CO2
Doubling time : ca. 50 hours
Harvest : cell harvest of ca. 10-15 x 106 cells/175 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint : genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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