| 产品名称: | ETCC-016 |
|---|---|
| 商品货号: | TS260342 |
| <strong>Cell line </strong>: | ETCC-016 |
| DSMZ no. : | TS260342 |
| Species : | confirmed as human by STR-typing |
| Cell type : | lung adenocarcinoma |
| Origin : | established in 2011 from the pleural effusion of a 53-year-old man of Singapore Chinese origin with advanced adenocarcinoma of the lung |
| Reference(s) : | 18126 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelioid cells growing in monolayers ; image |
| Medium : | 90% Dulbecco´s MEM + 10% h.i. FBS |
| Subculture : | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:3 - 1:4 every second day using trypsin/EDTA |
| Incubation : | at 37 °C with 10% CO2 |
| Doubling time : | ca. 50 hours |
| Harvest : | cell harvest of ca. 10-15 x 106 cells/175 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| 产品名称: | ETCC-016 |
|---|---|
| 商品货号: | TS260342 |
| <strong>Cell line </strong>: | ETCC-016 |
| DSMZ no. : | TS260342 |
| Species : | confirmed as human by STR-typing |
| Cell type : | lung adenocarcinoma |
| Origin : | established in 2011 from the pleural effusion of a 53-year-old man of Singapore Chinese origin with advanced adenocarcinoma of the lung |
| Reference(s) : | 18126 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelioid cells growing in monolayers ; image |
| Medium : | 90% Dulbecco´s MEM + 10% h.i. FBS |
| Subculture : | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:3 - 1:4 every second day using trypsin/EDTA |
| Incubation : | at 37 °C with 10% CO2 |
| Doubling time : | ca. 50 hours |
| Harvest : | cell harvest of ca. 10-15 x 106 cells/175 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |