| 产品名称: | U-2946 |
|---|---|
| 商品货号: | TS260344 |
| <strong>Cell line </strong>: | U-2946 |
| DSMZ no. : | TS260344 |
| Species : | confirmed as human by STR-typing |
| Cell type : | B cell lymphoma |
| Origin : | established in 1996 from the pleural effusion of a 52-year-old man with diffuse large B cell lymphoma (DLBCL). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) : | 18124, 18137, 18187 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | round cells growing singly or in small clumps in suspension; image; image |
| Medium : | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture : | seed out at ca. 0.1-0.3 x 106 cells/ml, optimal split ratio 1:5 - 1:10 every 3-4 days |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 20-30 hours |
| Harvest : | maximum density at ca. 1.5 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | CD3 -, CD5 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint : | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Cytogenetics : | human hyperdiploid karyotype; -47(43-48)<2n>XY, +7, der(8)del(8)(p22.2)t(8:14)(q24;q32), der(9)t(2;9)(p25:p21), der(14)t(8;14)(q24;q32), der(17)del(17)(p11.2)del(17)(q21q22); sdl with del(9)(q11); carries t(8;14) effecting IGH-MYC rearrangement; der(17) carries 1q21-derived focal amplicon inserted at 17p11 effecting MCL1 activation |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| 产品名称: | U-2946 |
|---|---|
| 商品货号: | TS260344 |
| <strong>Cell line </strong>: | U-2946 |
| DSMZ no. : | TS260344 |
| Species : | confirmed as human by STR-typing |
| Cell type : | B cell lymphoma |
| Origin : | established in 1996 from the pleural effusion of a 52-year-old man with diffuse large B cell lymphoma (DLBCL). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) : | 18124, 18137, 18187 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | round cells growing singly or in small clumps in suspension; image; image |
| Medium : | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture : | seed out at ca. 0.1-0.3 x 106 cells/ml, optimal split ratio 1:5 - 1:10 every 3-4 days |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 20-30 hours |
| Harvest : | maximum density at ca. 1.5 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | CD3 -, CD5 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint : | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Cytogenetics : | human hyperdiploid karyotype; -47(43-48)<2n>XY, +7, der(8)del(8)(p22.2)t(8:14)(q24;q32), der(9)t(2;9)(p25:p21), der(14)t(8;14)(q24;q32), der(17)del(17)(p11.2)del(17)(q21q22); sdl with del(9)(q11); carries t(8;14) effecting IGH-MYC rearrangement; der(17) carries 1q21-derived focal amplicon inserted at 17p11 effecting MCL1 activation |
| Viruses : | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |