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U-2946
U-2946
规格:
货期:
编号:TS260344
品牌:Testobio
产品名称: U-2946
商品货号: TS260344
<strong>Cell line </strong>: U-2946
DSMZ no. : TS260344
Species : confirmed as human by STR-typing
Cell type : B cell lymphoma
Origin : established in 1996 from the pleural effusion of a 52-year-old man with diffuse large B cell lymphoma (DLBCL). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)
Reference(s) : 18124, 18137, 18187
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: round cells growing singly or in small clumps in suspension; image; image
Medium : 90% RPMI 1640 + 10% h.i. FBS
Subculture : seed out at ca. 0.1-0.3 x 106 cells/ml, optimal split ratio 1:5 - 1:10 every 3-4 days
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 20-30 hours
Harvest : maximum density at ca. 1.5 x 106 cells/ml
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : CD3 -, CD5 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image
Fingerprint : genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database
Cytogenetics : human hyperdiploid karyotype; -47(43-48)<2n>XY, +7, der(8)del(8)(p22.2)t(8:14)(q24;q32), der(9)t(2;9)(p25:p21), der(14)t(8;14)(q24;q32), der(17)del(17)(p11.2)del(17)(q21q22); sdl with del(9)(q11); carries t(8;14) effecting IGH-MYC rearrangement; der(17) carries 1q21-derived focal amplicon inserted at 17p11 effecting MCL1 activation
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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U-2946

  • 货号: TS260344
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  • 品牌 : TESTOBIO
产品名称: U-2946
商品货号: TS260344
<strong>Cell line </strong>: U-2946
DSMZ no. : TS260344
Species : confirmed as human by STR-typing
Cell type : B cell lymphoma
Origin : established in 1996 from the pleural effusion of a 52-year-old man with diffuse large B cell lymphoma (DLBCL). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)
Reference(s) : 18124, 18137, 18187
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: round cells growing singly or in small clumps in suspension; image; image
Medium : 90% RPMI 1640 + 10% h.i. FBS
Subculture : seed out at ca. 0.1-0.3 x 106 cells/ml, optimal split ratio 1:5 - 1:10 every 3-4 days
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 20-30 hours
Harvest : maximum density at ca. 1.5 x 106 cells/ml
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Immunology : CD3 -, CD5 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image
Fingerprint : genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database
Cytogenetics : human hyperdiploid karyotype; -47(43-48)<2n>XY, +7, der(8)del(8)(p22.2)t(8:14)(q24;q32), der(9)t(2;9)(p25:p21), der(14)t(8;14)(q24;q32), der(17)del(17)(p11.2)del(17)(q21q22); sdl with del(9)(q11); carries t(8;14) effecting IGH-MYC rearrangement; der(17) carries 1q21-derived focal amplicon inserted at 17p11 effecting MCL1 activation
Viruses : PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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