MBU-TS1
MBU-TS1
规格:
货期:
编号:TS260349
品牌:Testobio
| 产品名称： | MBU-TS1 |
|---|---|
| 商品货号： | TS260349 |
| <strong>Cell line </strong>： | MBU-TS1 |
| DSMZ no. ： | TS260349 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | keratinocytes |
| Origin ： | established in 2011 from primary keratinocytes isolated from neonatal foreskin, transduced with retroviral vectors containing human telomerase and early SV40 region; carries additionally a FLAG epitope-tagged HRAS(V12) linked to IRES-driven GFP expression; MBU-TS1 is a xenograft-derived side line of MBU-T in Swiss nude mice |
| Reference(s) ： | 25648 |
| Biosafety level ： | 2, GMO-S2 |
| Risk assessment ： | Primary keratinocyte cells isolated from a neonatal foreskin were transduced with replication deficient amphotropic retroviral vectors containing a) the catalytic subunit of the human telomerase reverse transcriptase hTERT, b) SV40 early region, and c) constitutively active mutated HRASV12 genes for immortalization. Mutated HRAS exhibits strong oncogenic potential and is assigned to biosafety level 2. |
| Permissions and restrictions ： | A, B, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing in monolayers; image; image |
| Medium ： | 100% Keratinocyte SFM + 0.2 ng/ml hEGF + 15 µg/ml pituitary extract (no FBS added) |
| Subculture ： | seed out at ca. 0.5 x 106 cells/80 cm2 flask; split 1:2 every 8-10 days using Accutase (rinse two times with PBS and incubate for about 15 min at 37°C) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 100-140 hours |
| Harvest ： | cell harvest of ca. 2 x 106 cells/175 cm2 |
| Storage ： | frozen with 90% Keratinocyte SFM, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Fingerprint ： | fluorescent nonaplex PCR revealed the same STR DNA profile as MBU-IM, MBU-T, and MBU-TS4 |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV + |
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朱静
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李晴
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李晶晶
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周雪
13372109301
| 产品名称： | MBU-TS1 |
|---|---|
| 商品货号： | TS260349 |
| <strong>Cell line </strong>： | MBU-TS1 |
| DSMZ no. ： | TS260349 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | keratinocytes |
| Origin ： | established in 2011 from primary keratinocytes isolated from neonatal foreskin, transduced with retroviral vectors containing human telomerase and early SV40 region; carries additionally a FLAG epitope-tagged HRAS(V12) linked to IRES-driven GFP expression; MBU-TS1 is a xenograft-derived side line of MBU-T in Swiss nude mice |
| Reference(s) ： | 25648 |
| Biosafety level ： | 2, GMO-S2 |
| Risk assessment ： | Primary keratinocyte cells isolated from a neonatal foreskin were transduced with replication deficient amphotropic retroviral vectors containing a) the catalytic subunit of the human telomerase reverse transcriptase hTERT, b) SV40 early region, and c) constitutively active mutated HRASV12 genes for immortalization. Mutated HRAS exhibits strong oncogenic potential and is assigned to biosafety level 2. |
| Permissions and restrictions ： | A, B, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing in monolayers; image; image |
| Medium ： | 100% Keratinocyte SFM + 0.2 ng/ml hEGF + 15 µg/ml pituitary extract (no FBS added) |
| Subculture ： | seed out at ca. 0.5 x 106 cells/80 cm2 flask; split 1:2 every 8-10 days using Accutase (rinse two times with PBS and incubate for about 15 min at 37°C) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 100-140 hours |
| Harvest ： | cell harvest of ca. 2 x 106 cells/175 cm2 |
| Storage ： | frozen with 90% Keratinocyte SFM, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Fingerprint ： | fluorescent nonaplex PCR revealed the same STR DNA profile as MBU-IM, MBU-T, and MBU-TS4 |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV + |
