170-MG-BA
170-MG-BA
规格:
货期:
编号:TS260350
品牌:Testobio
| 产品名称： | 170-MG-BA |
|---|---|
| 商品货号： | TS260350 |
| <strong>Cell line </strong>： | 170-MG-BA |
| DSMZ no. ： | TS260350 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | glioblastoma |
| Origin ： | etablished in 2015 from the temporal lobe brain tumor diagnosed as glioblastoma multiforme of a 74-year-old Caucasian woman |
| Reference(s) ： | 18178 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in monolayers; image; image ; image |
| Medium ： | 90% MEM (with Earl´s salts) + 10% h.i. FBS + 2 mM L-glutamine + non-essential amino acids |
| Subculture ： | seed out at ca. 0.5-1 x 106 cells/175 cm2 flask; split 1:3 to 1:6 every 3-7 days using trypsin/EDTA |
| Incubation ： | at 37 °C without CO2 (keep lid of flask closed) |
| Doubling time ： | ca. 40-60 hours |
| Harvest ： | cell harvest of ca. 5-11 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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章丽敏
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朱静
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李晴
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李晶晶
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周雪
13372109301
| 产品名称： | 170-MG-BA |
|---|---|
| 商品货号： | TS260350 |
| <strong>Cell line </strong>： | 170-MG-BA |
| DSMZ no. ： | TS260350 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | glioblastoma |
| Origin ： | etablished in 2015 from the temporal lobe brain tumor diagnosed as glioblastoma multiforme of a 74-year-old Caucasian woman |
| Reference(s) ： | 18178 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in monolayers; image; image ; image |
| Medium ： | 90% MEM (with Earl´s salts) + 10% h.i. FBS + 2 mM L-glutamine + non-essential amino acids |
| Subculture ： | seed out at ca. 0.5-1 x 106 cells/175 cm2 flask; split 1:3 to 1:6 every 3-7 days using trypsin/EDTA |
| Incubation ： | at 37 °C without CO2 (keep lid of flask closed) |
| Doubling time ： | ca. 40-60 hours |
| Harvest ： | cell harvest of ca. 5-11 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
