JVE-103
JVE-103
规格:
货期:
编号:TS260360
品牌:Testobio
| 产品名称： | JVE-103 |
|---|---|
| 商品货号： | TS260360 |
| <strong>Cell line </strong>： | JVE-103 |
| DSMZ no. ： | TS260360 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | colon carcinoma |
| Origin ： | established from a liver metastasis of a 51-year-old man with colorectal adenocarcinoma (Duke stage D); cells were described in the literature to exhibit a homozygous deletion of the SMAD3 gene |
| Reference(s) ： | 18128, 18149 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in mono- and multilayered clusters; however, cells do not grow to complete confluency; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split culture 1:2 to 1:5 every two weeks using trypsin/EDTA (we recommend to wash the cells at least two times with PBS and incubate with trypsin for a minimum of 5 min.); the cells need 3-5 days for recovery and adherence after both thawing and trypsinization; cell number may be difficult to determine due to aggregate formation |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 10-14 days |
| Harvest ： | cell harvest of ca. 10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the original data set as published and unique by search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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| 产品名称： | JVE-103 |
|---|---|
| 商品货号： | TS260360 |
| <strong>Cell line </strong>： | JVE-103 |
| DSMZ no. ： | TS260360 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | colon carcinoma |
| Origin ： | established from a liver metastasis of a 51-year-old man with colorectal adenocarcinoma (Duke stage D); cells were described in the literature to exhibit a homozygous deletion of the SMAD3 gene |
| Reference(s) ： | 18128, 18149 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in mono- and multilayered clusters; however, cells do not grow to complete confluency; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split culture 1:2 to 1:5 every two weeks using trypsin/EDTA (we recommend to wash the cells at least two times with PBS and incubate with trypsin for a minimum of 5 min.); the cells need 3-5 days for recovery and adherence after both thawing and trypsinization; cell number may be difficult to determine due to aggregate formation |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 10-14 days |
| Harvest ： | cell harvest of ca. 10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the original data set as published and unique by search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
