KP-363T
KP-363T
规格:
货期:
编号:TS260361
品牌:Testobio
| 产品名称： | KP-363T |
|---|---|
| 商品货号： | TS260361 |
| <strong>Cell line </strong>： | KP-363T |
| DSMZ no. ： | TS260361 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | colon carcinoma |
| Origin ： | established from the tumor mass of an 80-year-old man with colorectal adenocarcinoma (Dukes stage B2) from the left side; described in the literature to carry mutations in the BRAF, PIK3CA, and TP53 genes, a deletion in the SMAD4 gene, and hypermethylation of the CDKN2A promoter |
| Reference(s) ： | 18128 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently as monolayer and in densely packed islets; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:5 once a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of up to 80 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the original data set as published and unique by search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
KP-363T
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | KP-363T |
|---|---|
| 商品货号： | TS260361 |
| <strong>Cell line </strong>： | KP-363T |
| DSMZ no. ： | TS260361 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | colon carcinoma |
| Origin ： | established from the tumor mass of an 80-year-old man with colorectal adenocarcinoma (Dukes stage B2) from the left side; described in the literature to carry mutations in the BRAF, PIK3CA, and TP53 genes, a deletion in the SMAD4 gene, and hypermethylation of the CDKN2A promoter |
| Reference(s) ： | 18128 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently as monolayer and in densely packed islets; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:5 once a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of up to 80 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the original data set as published and unique by search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
