JVE-059
JVE-059
规格:
货期:
编号:TS260363
品牌:Testobio
| 产品名称： | JVE-059 |
|---|---|
| 商品货号： | TS260363 |
| <strong>Cell line </strong>： | JVE-059 |
| DSMZ no. ： | TS260363 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | colon carcinoma |
| Origin ： | established from the tumor mass of a 58-year-old man with colorectal adenocarcinoma of the colon transversum (Duke stage D); cells were described in the literature to exhibit microsatellite instability (MSI-H) and a partial methylation of the CDKN2A promoter |
| Reference(s) ： | 18128, 18149 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round to oval cells, growing strongly adherent, singly and in islets in monolayers; image; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/25 cm2 flask; split confluent culture 1:2 once or twice a week using trypsin/EDTA (we recommend to wash the cells at least two times with PBS and incubate with trypsin for a minimum of 5 min.); avoid a long thawing procedure to prevent a decrease in viability |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-4 days |
| Harvest ： | cell harvest of ca. 10 x 106 cells/25 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the original data set as published and unique by search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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周雪
13372109301
| 产品名称： | JVE-059 |
|---|---|
| 商品货号： | TS260363 |
| <strong>Cell line </strong>： | JVE-059 |
| DSMZ no. ： | TS260363 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | colon carcinoma |
| Origin ： | established from the tumor mass of a 58-year-old man with colorectal adenocarcinoma of the colon transversum (Duke stage D); cells were described in the literature to exhibit microsatellite instability (MSI-H) and a partial methylation of the CDKN2A promoter |
| Reference(s) ： | 18128, 18149 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round to oval cells, growing strongly adherent, singly and in islets in monolayers; image; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/25 cm2 flask; split confluent culture 1:2 once or twice a week using trypsin/EDTA (we recommend to wash the cells at least two times with PBS and incubate with trypsin for a minimum of 5 min.); avoid a long thawing procedure to prevent a decrease in viability |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-4 days |
| Harvest ： | cell harvest of ca. 10 x 106 cells/25 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the original data set as published and unique by search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
