538-MG-BA
538-MG-BA
规格:
货期:
编号:TS260365
品牌:Testobio
| 产品名称： | 538-MG-BA |
|---|---|
| 商品货号： | TS260365 |
| <strong>Cell line </strong>： | 538-MG-BA |
| DSMZ no. ： | TS260365 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | glioblastoma |
| Origin ： | etablished in 2015 from the brain tumor diagnosed as glioblastoma multiforme of a 49-year-old Caucasian man |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large cells growing adherently in monolayers; image; image; image |
| Medium ： | 90% MEM (with Earl´s salts) + 10% h.i. FBS + 2 mM L-glutamine + non-essential amino acids |
| Subculture ： | seed out at ca. 1 x 106 cells/175 cm2 flask; split 1:6 to 1:8 every 4-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-35 hours |
| Harvest ： | cell harvest of ca. 8-10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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538-MG-BA
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | 538-MG-BA |
|---|---|
| 商品货号： | TS260365 |
| <strong>Cell line </strong>： | 538-MG-BA |
| DSMZ no. ： | TS260365 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | glioblastoma |
| Origin ： | etablished in 2015 from the brain tumor diagnosed as glioblastoma multiforme of a 49-year-old Caucasian man |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large cells growing adherently in monolayers; image; image; image |
| Medium ： | 90% MEM (with Earl´s salts) + 10% h.i. FBS + 2 mM L-glutamine + non-essential amino acids |
| Subculture ： | seed out at ca. 1 x 106 cells/175 cm2 flask; split 1:6 to 1:8 every 4-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-35 hours |
| Harvest ： | cell harvest of ca. 8-10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
