CCC-5
CCC-5
规格:
货期:
编号:TS260376
品牌:Testobio
| 产品名称： | CCC-5 |
|---|---|
| 商品货号： | TS260376 |
| <strong>Cell line </strong>： | CCC-5 |
| DSMZ no. ： | TS260376 |
| Species ： | human (Homo sapiens) |
| Cell type ： | cholangiocellular carcinoma |
| Origin ： | established in 2015 from the malignant pleural effusion of a distal cholangiocellular carcinoma of a 59-year-old Caucasian man; cells were described in the literature to carry several mutations in oncogenes and tumor suppressor genes (see ref.) |
| Reference(s) ： | 18142 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in monolayers in a cobblestone pattern; image |
| Medium ： | 80% mixture of DMEM and Keratinocyte SFM (at 2:1) + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:5 once or twice a week using trypsin/EDTA for about 10 min |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 2-3 days |
| Harvest ： | cell harvest of 5-8 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Plasmocin, then negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the original data set as published and unique by search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
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周雪
13372109301
| 产品名称： | CCC-5 |
|---|---|
| 商品货号： | TS260376 |
| <strong>Cell line </strong>： | CCC-5 |
| DSMZ no. ： | TS260376 |
| Species ： | human (Homo sapiens) |
| Cell type ： | cholangiocellular carcinoma |
| Origin ： | established in 2015 from the malignant pleural effusion of a distal cholangiocellular carcinoma of a 59-year-old Caucasian man; cells were described in the literature to carry several mutations in oncogenes and tumor suppressor genes (see ref.) |
| Reference(s) ： | 18142 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in monolayers in a cobblestone pattern; image |
| Medium ： | 80% mixture of DMEM and Keratinocyte SFM (at 2:1) + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:5 once or twice a week using trypsin/EDTA for about 10 min |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 2-3 days |
| Harvest ： | cell harvest of 5-8 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Plasmocin, then negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the original data set as published and unique by search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
