GH
GH
规格:
货期:
编号:TS260377
品牌:Testobio
| 产品名称： | GH |
|---|---|
| 商品货号： | TS260377 |
| <strong>Cell line </strong>： | GH |
| DSMZ no. ： | TS260377 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | testicular germ cell tumor |
| Origin ： | established in 1980 from a human testicular teratocarcinoma of a male patient of unknown age; cells are described in the literature to express mRNA for endogenous retrovirus and virus-like particles; they are further described in the literature to be ultrasensitive to cisplatin |
| Reference(s) ： | 18139, 18140, 18141, 15195 |
| Biosafety level ： | 2 |
| Risk assessment ： | As described in literature, the GH cells produce human teratocarcinoma derived viruses (HDTV) which are identical to human endogenous retrovirus K (HERV-K). HERV-K is assigned to biosafety level 2 in Germany. The cell culture supernatant shows a marginal reverse transcriptase activity in the cell culture supernatant which can slightly be stimulated by 5-azacytidine and strongly applying 5-iodo-2-deoxyuridine. The activity was determined employing the product enhanced reverse transcriptase assay (PERT) |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polygonal cells growing adherently in monolayers and discrete colonies; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/175 cm2 flask; split confluent culture 1:2 - 1:4 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 8 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV-, HERV-K + |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | GH |
|---|---|
| 商品货号： | TS260377 |
| <strong>Cell line </strong>： | GH |
| DSMZ no. ： | TS260377 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | testicular germ cell tumor |
| Origin ： | established in 1980 from a human testicular teratocarcinoma of a male patient of unknown age; cells are described in the literature to express mRNA for endogenous retrovirus and virus-like particles; they are further described in the literature to be ultrasensitive to cisplatin |
| Reference(s) ： | 18139, 18140, 18141, 15195 |
| Biosafety level ： | 2 |
| Risk assessment ： | As described in literature, the GH cells produce human teratocarcinoma derived viruses (HDTV) which are identical to human endogenous retrovirus K (HERV-K). HERV-K is assigned to biosafety level 2 in Germany. The cell culture supernatant shows a marginal reverse transcriptase activity in the cell culture supernatant which can slightly be stimulated by 5-azacytidine and strongly applying 5-iodo-2-deoxyuridine. The activity was determined employing the product enhanced reverse transcriptase assay (PERT) |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polygonal cells growing adherently in monolayers and discrete colonies; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/175 cm2 flask; split confluent culture 1:2 - 1:4 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 8 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV-, HERV-K + |
