JVE-187
JVE-187
规格:
货期:
编号:TS260384
品牌:Testobio
| 产品名称： | JVE-187 |
|---|---|
| 商品货号： | TS260384 |
| <strong>Cell line </strong>： | JVE-187 |
| DSMZ no. ： | TS260384 |
| Species ： | human (Homo sapiens) |
| Cell type ： | colon carcinoma |
| Origin ： | established in 2015 from the liver metastasis of a colorectal adenocarcinoma (Duke stage D) of a 60-year-old caucasian woman; cells were described in literature to carry mutations in the KRAS, PIK3CA, and SMAD4 genes, and hypermethylation of the CDKN2A and MGMT promoter |
| Reference(s) ： | 18128, 18149 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells growing as monolayer and some multilayered clusters; after thawing cells look blistered; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:3 to 1:5 once a week using trypsin/EDTA for at least 10 min. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-5 days |
| Harvest ： | cell harvest of ca. 10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the original data set as published and unique by search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |
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周雪
13372109301
| 产品名称： | JVE-187 |
|---|---|
| 商品货号： | TS260384 |
| <strong>Cell line </strong>： | JVE-187 |
| DSMZ no. ： | TS260384 |
| Species ： | human (Homo sapiens) |
| Cell type ： | colon carcinoma |
| Origin ： | established in 2015 from the liver metastasis of a colorectal adenocarcinoma (Duke stage D) of a 60-year-old caucasian woman; cells were described in literature to carry mutations in the KRAS, PIK3CA, and SMAD4 genes, and hypermethylation of the CDKN2A and MGMT promoter |
| Reference(s) ： | 18128, 18149 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells growing as monolayer and some multilayered clusters; after thawing cells look blistered; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:3 to 1:5 once a week using trypsin/EDTA for at least 10 min. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-5 days |
| Harvest ： | cell harvest of ca. 10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the original data set as published and unique by search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |
