| 产品名称: | CX-03 |
|---|---|
| 商品货号: | TS260385 |
| <strong>Cell line </strong>: | CX-03 |
| DSMZ no. : | TS260385 |
| Species : | human (Homo sapiens) |
| Cell type : | uterine carcinosarcoma |
| Origin : | established in 2015 after xenotransplantation in SCID-mice of primary tumor cells from a mixed Muellerian tumor (Carcinosarcoma) of the uterus of a 49-year-old Caucasian woman; cells are described in literature to produce mesothelin and mucus |
| Reference(s) : | 18179 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelioid cells growing adherently in monolayers; image; image |
| Medium : | 80% Dulbecco´s MEM + 20% h.i. FBS |
| Subculture : | seed out at ca. 0.5 x 106 cells/80 cm2 flask; split confluent culture 1:2 -1:4 every 7 days using trypsin/EDTA; cells grow rather slowly in the first two week after thawing |
| Incubation : | at 37 °C with 10% CO2 |
| Doubling time : | ca. 4-7 days |
| Harvest : | cell harvest of ca. 4 x 106 cells/175 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |
| 产品名称: | CX-03 |
|---|---|
| 商品货号: | TS260385 |
| <strong>Cell line </strong>: | CX-03 |
| DSMZ no. : | TS260385 |
| Species : | human (Homo sapiens) |
| Cell type : | uterine carcinosarcoma |
| Origin : | established in 2015 after xenotransplantation in SCID-mice of primary tumor cells from a mixed Muellerian tumor (Carcinosarcoma) of the uterus of a 49-year-old Caucasian woman; cells are described in literature to produce mesothelin and mucus |
| Reference(s) : | 18179 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelioid cells growing adherently in monolayers; image; image |
| Medium : | 80% Dulbecco´s MEM + 20% h.i. FBS |
| Subculture : | seed out at ca. 0.5 x 106 cells/80 cm2 flask; split confluent culture 1:2 -1:4 every 7 days using trypsin/EDTA; cells grow rather slowly in the first two week after thawing |
| Incubation : | at 37 °C with 10% CO2 |
| Doubling time : | ca. 4-7 days |
| Harvest : | cell harvest of ca. 4 x 106 cells/175 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in PCR assay |
| Immunology : | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint : | genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database |
| Viruses : | PCR: EBV -, HBV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |