JVE-222
JVE-222
规格:
货期:
编号:TS260390
品牌:Testobio
| 产品名称： | JVE-222 |
|---|---|
| 商品货号： | TS260390 |
| <strong>Cell line </strong>： | JVE-222 |
| DSMZ no. ： | TS260390 |
| Species ： | human (Homo sapiens) |
| Cell type ： | colon carcinoma |
| Origin ： | established from the primary tissue of an ascending colorectal adenocarcinoma (Dukes stage B2) of a 60-year-old woman; cells were described in literature to carry mutations in the KRAS, PIK3CA, and APC genes, a partial methylation of the CDKN2A promoter and to display microsatellite instability (MSI-H) |
| Reference(s) ： | 18128, 18149 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in islets and monolayers; culture may contain cell debris; image; image; image; image |
| Medium ： | 90% mixture of Hams F12 + Dulbeccos MEM (1:1) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/25 cm2; split conflent culture 1:2 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70 hours |
| Harvest ： | cell harvest of 25-30 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the original data set as published and unique by search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |
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| 产品名称： | JVE-222 |
|---|---|
| 商品货号： | TS260390 |
| <strong>Cell line </strong>： | JVE-222 |
| DSMZ no. ： | TS260390 |
| Species ： | human (Homo sapiens) |
| Cell type ： | colon carcinoma |
| Origin ： | established from the primary tissue of an ascending colorectal adenocarcinoma (Dukes stage B2) of a 60-year-old woman; cells were described in literature to carry mutations in the KRAS, PIK3CA, and APC genes, a partial methylation of the CDKN2A promoter and to display microsatellite instability (MSI-H) |
| Reference(s) ： | 18128, 18149 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in islets and monolayers; culture may contain cell debris; image; image; image; image |
| Medium ： | 90% mixture of Hams F12 + Dulbeccos MEM (1:1) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/25 cm2; split conflent culture 1:2 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70 hours |
| Harvest ： | cell harvest of 25-30 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the original data set as published and unique by search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |
