JVE-371
JVE-371
规格:
货期:
编号:TS260392
品牌:Testobio
| 产品名称： | JVE-371 |
|---|---|
| 商品货号： | TS260392 |
| <strong>Cell line </strong>： | JVE-371 |
| DSMZ no. ： | TS260392 |
| Species ： | human (Homo sapiens) |
| Cell type ： | colon carcinoma |
| Origin ： | established from the liver metastasis of a malignant colorectal adenocarcinoma (Duke stage D) of a 67-year-old man; cells were described in literature to carry mutations in the KRAS, PIK3CA and TP53 genes but no methylation of the CDKN2A promoter |
| Reference(s) ： | 18128, 18149 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in monolayers and in densely packed islets, sometimes in multilayered clusters with cell-free "calderas" or bubbles; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 once a week using trypsin/EDTA for 10-15 min |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-5 days |
| Harvest ： | cell harvest of 8-10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the original data set as published and unique by search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
JVE-371
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | JVE-371 |
|---|---|
| 商品货号： | TS260392 |
| <strong>Cell line </strong>： | JVE-371 |
| DSMZ no. ： | TS260392 |
| Species ： | human (Homo sapiens) |
| Cell type ： | colon carcinoma |
| Origin ： | established from the liver metastasis of a malignant colorectal adenocarcinoma (Duke stage D) of a 67-year-old man; cells were described in literature to carry mutations in the KRAS, PIK3CA and TP53 genes but no methylation of the CDKN2A promoter |
| Reference(s) ： | 18128, 18149 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in monolayers and in densely packed islets, sometimes in multilayered clusters with cell-free "calderas" or bubbles; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 once a week using trypsin/EDTA for 10-15 min |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-5 days |
| Harvest ： | cell harvest of 8-10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the original data set as published and unique by search of the international STR database |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |
