KMST-6-TNF
KMST-6-TNF
规格:
货期:
编号:TS260395
品牌:Testobio
| 产品名称： | KMST-6-TNF |
|---|---|
| 商品货号： | TS260395 |
| <strong>Cell line </strong>： | KMST-6-TNF |
| DSMZ no. ： | TS260395 |
| Species ： | human (Homo sapiens) |
| Cell type ： | fibroblasts |
| Origin ： | Immortalized human fibroblasts established in 1982 by Gamma-irradiation of isolated fibroblasts from a 9-week old female fetus (KMST-6). Subsequent transformation with a plasmid containing human TNF-alpha cDNA confering TNF production and hygromycin resistance |
| Reference(s) ： | 18150, 18151 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | The original cells (KMST-6) were transfected with a plasmid (pCDL-81) containing the cDNA for TNFalpha and selected via hygromycin resistence. Neither the cells nor TNFa present a danger for humans and thus the cell line is classified as S1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblastoid cells growing as monolayer; image; image; image |
| Medium ： | 90% MEM (with Earle salts) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:3 to 1:4 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | ca. 4-5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the data set as from JCRB and unique by search of the international STR database |
| Cytogenetics ： | is described by Namba et al. to carry structural and numerical chromosomal aberrations |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |
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13372109301
| 产品名称： | KMST-6-TNF |
|---|---|
| 商品货号： | TS260395 |
| <strong>Cell line </strong>： | KMST-6-TNF |
| DSMZ no. ： | TS260395 |
| Species ： | human (Homo sapiens) |
| Cell type ： | fibroblasts |
| Origin ： | Immortalized human fibroblasts established in 1982 by Gamma-irradiation of isolated fibroblasts from a 9-week old female fetus (KMST-6). Subsequent transformation with a plasmid containing human TNF-alpha cDNA confering TNF production and hygromycin resistance |
| Reference(s) ： | 18150, 18151 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | The original cells (KMST-6) were transfected with a plasmid (pCDL-81) containing the cDNA for TNFalpha and selected via hygromycin resistence. Neither the cells nor TNFa present a danger for humans and thus the cell line is classified as S1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblastoid cells growing as monolayer; image; image; image |
| Medium ： | 90% MEM (with Earle salts) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:3 to 1:4 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | ca. 4-5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | short tandem repeat (STR) profile is identical to the data set as from JCRB and unique by search of the international STR database |
| Cytogenetics ： | is described by Namba et al. to carry structural and numerical chromosomal aberrations |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |
