CHP-100
CHP-100
规格:
货期:
编号:TS260403
品牌:Testobio
| 产品名称： | CHP-100 |
|---|---|
| 商品货号： | TS260403 |
| <strong>Cell line </strong>： | CHP-100 |
| DSMZ no. ： | TS260403 |
| Species ： | human |
| Cell type ： | neuroepithelioma |
| Origin ： | established from epidural tumor tissue of the spine obtained by thoracic laminectomy of a 12-year old girl with diagnosed neuroblastoma. Cell line was described in literature to be tumorigenic in nude mice and in a later analysis as neuroepithelioma. In 1983 two morphological sublines, CHP-100-L and -S were described. |
| Reference(s) ： | 18163, 18164, 18165, 18166, 18167 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently as monolayer; only very few cells exhibit a neuronal-like morphology; image; image; image |
| Medium ： | 90% McCoys 5A + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:4 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 36 hours |
| Harvest ： | cell harvest of 10-15 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR revealed a unique STR DNA profile and identical to the 2014 published data of the corresponding cell line CHP-100-L |
| Molec. Genetics ： | expression of fusion gene EWS-FLI1 was confirmed by RT-PCR |
| Viruses ： | PCR: HBV -, HCV -, EBV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV + |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
CHP-100
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | CHP-100 |
|---|---|
| 商品货号： | TS260403 |
| <strong>Cell line </strong>： | CHP-100 |
| DSMZ no. ： | TS260403 |
| Species ： | human |
| Cell type ： | neuroepithelioma |
| Origin ： | established from epidural tumor tissue of the spine obtained by thoracic laminectomy of a 12-year old girl with diagnosed neuroblastoma. Cell line was described in literature to be tumorigenic in nude mice and in a later analysis as neuroepithelioma. In 1983 two morphological sublines, CHP-100-L and -S were described. |
| Reference(s) ： | 18163, 18164, 18165, 18166, 18167 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently as monolayer; only very few cells exhibit a neuronal-like morphology; image; image; image |
| Medium ： | 90% McCoys 5A + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:4 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 36 hours |
| Harvest ： | cell harvest of 10-15 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR revealed a unique STR DNA profile and identical to the 2014 published data of the corresponding cell line CHP-100-L |
| Molec. Genetics ： | expression of fusion gene EWS-FLI1 was confirmed by RT-PCR |
| Viruses ： | PCR: HBV -, HCV -, EBV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV + |
