U2OS#18
U2OS#18
规格:
货期:
编号:TS260414
品牌:Testobio
| 产品名称： | U2OS#18 |
|---|---|
| 商品货号： | TS260414 |
| <strong>Cell line </strong>： | U2OS#18 |
| DSMZ no. ： | TS260414 |
| Species ： | human |
| Cell type ： | osteosarcoma |
| Origin ： | Genetic modification of human osteosarcoma cell line U-2-OS for studying sister chromatid recombination (SCR). The cell line is described to contain a single copy of the SCR reporter inserted as nested intron and carries the GFP-Gene and is puromycin resistant. A series of modified cell lines with gene disruptions in RAD51 paralogs are also available (DSMZ ACC 833-ACC 842). |
| Reference(s) ： | 24159, 18169 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently as monolayer; image; image |
| Medium ： | 90% Dulbecco´s MEM + 10% h.i. FBS + (additionally) 2 mM L-glutamine; (to verify puromycin resistance, 1 µg/ml should be added) |
| Subculture ： | seed out at 1 x 106 cells/80 cm2; split confluent culture once or twice a week 1:3 to 1:10 using trypsin/EDTA; apparently confluent cultures can be further cultivated for 2-3 days to increase the maximal cell harvest |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | cell harvest of 5-10 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR revealed an authentic STR DNA profile as U-2-OS (DSMZ ACC 785) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |
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章丽敏
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朱静
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李晴
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李晶晶
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周雪
13372109301
| 产品名称： | U2OS#18 |
|---|---|
| 商品货号： | TS260414 |
| <strong>Cell line </strong>： | U2OS#18 |
| DSMZ no. ： | TS260414 |
| Species ： | human |
| Cell type ： | osteosarcoma |
| Origin ： | Genetic modification of human osteosarcoma cell line U-2-OS for studying sister chromatid recombination (SCR). The cell line is described to contain a single copy of the SCR reporter inserted as nested intron and carries the GFP-Gene and is puromycin resistant. A series of modified cell lines with gene disruptions in RAD51 paralogs are also available (DSMZ ACC 833-ACC 842). |
| Reference(s) ： | 24159, 18169 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently as monolayer; image; image |
| Medium ： | 90% Dulbecco´s MEM + 10% h.i. FBS + (additionally) 2 mM L-glutamine; (to verify puromycin resistance, 1 µg/ml should be added) |
| Subculture ： | seed out at 1 x 106 cells/80 cm2; split confluent culture once or twice a week 1:3 to 1:10 using trypsin/EDTA; apparently confluent cultures can be further cultivated for 2-3 days to increase the maximal cell harvest |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | cell harvest of 5-10 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR revealed an authentic STR DNA profile as U-2-OS (DSMZ ACC 785) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |
