U2OS#18-XRCC3-6A/X3+
U2OS#18-XRCC3-6A/X3+
规格:
货期:
编号:TS260431
品牌:Testobio
| 产品名称： | U2OS#18-XRCC3-6A/X3+ |
|---|---|
| 商品货号： | TS260431 |
| <strong>Cell line </strong>： | U2OS#18-XRCC3-6A/X3+ |
| DSMZ no. ： | TS260431 |
| Species ： | human |
| Cell type ： | osteosarcoma |
| Origin ： | Genetical modification of U2OS#18 (DSMZ ACC 832, a reporter cell line for studying sister chromatid recombination, SCR). This cell line belongs to the series of genetically disrupted RAD51 paralogs with reversion of the disruption by retroviral transfection with the functional gene (the non-complemented sister cell line is ACC 837). Cells are described in the literature to be puromycin and hygromycin resistant |
| Reference(s) ： | 18169, 18186 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently as monolayer; image; image; image |
| Medium ： | 90% Dulbecco´s MEM + 10% h.i. FBS + (additionally) 2 mM L-glutamine (to verify antibiotic resistance, 1 µg/ml puromycin and/or 150 µg/ml hygromycin should be added) |
| Subculture ： | seed out at 1 x 106 cells/80 cm2; split 1:10 to 1:20 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | cell harvest of ca. 14 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | standardized STR9 (ANSI/ASN-0002-2011) revealed an authentic STR DNA profile matching parental cell line U-2-OS (DSMZ ACC 785) |
| Molec. Genetics ： | frameshift mutations in exon 5 of XRCC3 were verified by sequence analysis and forced expression of XRCC3 in the retrovirally transfected cell line was confirmed by RQ-PCR analysis |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV1/2 -, MLV - |
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周雪
13372109301
| 产品名称： | U2OS#18-XRCC3-6A/X3+ |
|---|---|
| 商品货号： | TS260431 |
| <strong>Cell line </strong>： | U2OS#18-XRCC3-6A/X3+ |
| DSMZ no. ： | TS260431 |
| Species ： | human |
| Cell type ： | osteosarcoma |
| Origin ： | Genetical modification of U2OS#18 (DSMZ ACC 832, a reporter cell line for studying sister chromatid recombination, SCR). This cell line belongs to the series of genetically disrupted RAD51 paralogs with reversion of the disruption by retroviral transfection with the functional gene (the non-complemented sister cell line is ACC 837). Cells are described in the literature to be puromycin and hygromycin resistant |
| Reference(s) ： | 18169, 18186 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently as monolayer; image; image; image |
| Medium ： | 90% Dulbecco´s MEM + 10% h.i. FBS + (additionally) 2 mM L-glutamine (to verify antibiotic resistance, 1 µg/ml puromycin and/or 150 µg/ml hygromycin should be added) |
| Subculture ： | seed out at 1 x 106 cells/80 cm2; split 1:10 to 1:20 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | cell harvest of ca. 14 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | standardized STR9 (ANSI/ASN-0002-2011) revealed an authentic STR DNA profile matching parental cell line U-2-OS (DSMZ ACC 785) |
| Molec. Genetics ： | frameshift mutations in exon 5 of XRCC3 were verified by sequence analysis and forced expression of XRCC3 in the retrovirally transfected cell line was confirmed by RQ-PCR analysis |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV1/2 -, MLV - |
