CL-52b
CL-52b
规格:
货期:
编号:TS260434
品牌:Testobio
| 产品名称： | CL-52b |
|---|---|
| 商品货号： | TS260434 |
| <strong>Cell line </strong>： | CL-52b |
| DSMZ no. ： | TS260434 |
| Species ： | confirmed as Rattus Norwegicus origin by COI DNA barcoding |
| Cell type ： | hepatocellular carcinoma |
| Origin ： | established ca. 1986 from the liver of a diethylnitrosamine-treated male Wistar AF/Han rat. Described to exhibit chromosomal instability and to induce cholangiohepatocellular carcinomas in nude mice |
| Reference(s) ： | 14658, 18172 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells, some with spindle-like extensions growing adherently in monolayers and clusters; image; image |
| Medium ： | 80-90% MEM (with Earles salts) + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/175 cm2 flask; split confluent culture 1:3 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of 10-12 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | n.a. |
| Viruses ： | PCR: MLV - |
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周雪
13372109301
| 产品名称： | CL-52b |
|---|---|
| 商品货号： | TS260434 |
| <strong>Cell line </strong>： | CL-52b |
| DSMZ no. ： | TS260434 |
| Species ： | confirmed as Rattus Norwegicus origin by COI DNA barcoding |
| Cell type ： | hepatocellular carcinoma |
| Origin ： | established ca. 1986 from the liver of a diethylnitrosamine-treated male Wistar AF/Han rat. Described to exhibit chromosomal instability and to induce cholangiohepatocellular carcinomas in nude mice |
| Reference(s) ： | 14658, 18172 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells, some with spindle-like extensions growing adherently in monolayers and clusters; image; image |
| Medium ： | 80-90% MEM (with Earles salts) + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/175 cm2 flask; split confluent culture 1:3 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of 10-12 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | n.a. |
| Viruses ： | PCR: MLV - |
