HEK293-DR-GFP-RAD51B-9/B+
HEK293-DR-GFP-RAD51B-9/B+
规格:
货期:
编号:TS260441
品牌:Testobio
| 产品名称： | HEK293-DR-GFP-RAD51B-9/B+ |
|---|---|
| 商品货号： | TS260441 |
| <strong>Cell line </strong>： | HEK293-DR-GFP-RAD51B-9/B+ |
| DSMZ no. ： | TS260441 |
| Species ： | human (Homo sapiens) |
| Cell type ： | embryonal kidney |
| Origin ： | Genetic modification of HEK293-DR-GFP-RAD51B-9 (DSMZ ACC 845) which belongs to a series of genetically disrupted RAD51 paralogs. Here, in TS260441, the RAD51B gene is functionally reconstituted by retroviral transfection. Cells are described in the literature to be puromycin and hygromycin resistant |
| Reference(s) ： | 18173, 18186 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblastoid cells growing as monolayer, mostly with spike-like extensions; image; image |
| Medium ： | 90% Dulbecco´s MEM + 10% h.i. FBS + (additionally) 2 mM L-glutamine (to sustain genetic stability cells should be maintained, at least periodically, with 0.5 µg/ml puromycin and/or 100 µg/ml hygromycin) |
| Subculture ： | culture vessels should be coated with 0.01% poly-L-lysine solution; seed out at 1 x 106 cells/80 cm2; split subconfluent culture 1:5 to 1:10 every 2-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 24-36 hours |
| Harvest ： | cell harvest of 10-5 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Fingerprint ： | standardized STR9 (ANSI/ASN-0002-2011) revealed an authentic STR DNA profile matching parental cell line (HEK)293 (DSMZ ACC 305) |
| Molec. Genetics ： | frameshift mutation in exon 3 of RAD51B was verified by sequence analysis and forced expression of RAD51B in the retrovirally transfected cell line was confirmed by RQ-PCR analysis |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV1/2 -, XMLV - |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
HEK293-DR-GFP-RAD51B-9/B+
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | HEK293-DR-GFP-RAD51B-9/B+ |
|---|---|
| 商品货号： | TS260441 |
| <strong>Cell line </strong>： | HEK293-DR-GFP-RAD51B-9/B+ |
| DSMZ no. ： | TS260441 |
| Species ： | human (Homo sapiens) |
| Cell type ： | embryonal kidney |
| Origin ： | Genetic modification of HEK293-DR-GFP-RAD51B-9 (DSMZ ACC 845) which belongs to a series of genetically disrupted RAD51 paralogs. Here, in TS260441, the RAD51B gene is functionally reconstituted by retroviral transfection. Cells are described in the literature to be puromycin and hygromycin resistant |
| Reference(s) ： | 18173, 18186 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblastoid cells growing as monolayer, mostly with spike-like extensions; image; image |
| Medium ： | 90% Dulbecco´s MEM + 10% h.i. FBS + (additionally) 2 mM L-glutamine (to sustain genetic stability cells should be maintained, at least periodically, with 0.5 µg/ml puromycin and/or 100 µg/ml hygromycin) |
| Subculture ： | culture vessels should be coated with 0.01% poly-L-lysine solution; seed out at 1 x 106 cells/80 cm2; split subconfluent culture 1:5 to 1:10 every 2-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 24-36 hours |
| Harvest ： | cell harvest of 10-5 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Fingerprint ： | standardized STR9 (ANSI/ASN-0002-2011) revealed an authentic STR DNA profile matching parental cell line (HEK)293 (DSMZ ACC 305) |
| Molec. Genetics ： | frameshift mutation in exon 3 of RAD51B was verified by sequence analysis and forced expression of RAD51B in the retrovirally transfected cell line was confirmed by RQ-PCR analysis |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV1/2 -, XMLV - |
