NT-27a
NT-27a
规格:
货期:
编号:TS260458
品牌:Testobio
| 产品名称： | NT-27a |
|---|---|
| 商品货号： | TS260458 |
| <strong>Cell line </strong>： | NT-27a |
| DSMZ no. ： | TS260458 |
| Species ： | confirmed as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | hepatocellular tumor |
| Origin ： | subclone of NT-27 which was established ca. 1986 from the liver of a male Wistar AF/Han rat treated with N-methyl-N-nitrosourea |
| Reference(s) ： | 18184, 18185, 14658, 15145 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in clusters as monolayer; image; image |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/175 cm2; split confluent culture 1:5 to 1:7 once a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24-30 hours |
| Harvest ： | cell harvest of ca. 25 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Viruses ： | PCR: XMLV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | NT-27a |
|---|---|
| 商品货号： | TS260458 |
| <strong>Cell line </strong>： | NT-27a |
| DSMZ no. ： | TS260458 |
| Species ： | confirmed as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | hepatocellular tumor |
| Origin ： | subclone of NT-27 which was established ca. 1986 from the liver of a male Wistar AF/Han rat treated with N-methyl-N-nitrosourea |
| Reference(s) ： | 18184, 18185, 14658, 15145 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid cells growing adherently in clusters as monolayer; image; image |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/175 cm2; split confluent culture 1:5 to 1:7 once a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24-30 hours |
| Harvest ： | cell harvest of ca. 25 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Viruses ： | PCR: XMLV - |
