CHO-INSR-1284
CHO-INSR-1284
规格:
货期:
编号:TS260463
品牌:Testobio
| 产品名称： | CHO-INSR-1284 |
|---|---|
| 商品货号： | TS260463 |
| <strong>Cell line </strong>： | CHO-INSR-1284 |
| DSMZ no. ： | TS260463 |
| Species ： | confirmed as Cricetulus griseues (Chinese hamster) origin by COI DNA barcoding |
| Cell type ： | ovary cells |
| Origin ： | Flp-In derivate of CHO-K1 cell line (originated from an ovary biopsy of an adult Chinese hamster in 1957) which were transfected with the gene for human insulin receptor B (INSR) inserted in the vector pCDNA(TM)5-FRT-TO from Invitrogen |
| Reference(s) ： | 18190 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid-like cells growing adherently in monolayers; image; image; image |
| Medium ： | 90% Ham´s F12 + 10% h.i. FBS + 1 mM L-glutamine + 0.3 mg/ml Hygromycin |
| Subculture ： | seed out 0.5-1 x 106 cells/80cm2; split 1:4 to 1:6 once or twice a week using accutase; after thawing viability may decrease and cells may need a few days to recover |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 8-10 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD220 +; image |
| Viruses ： | PCR: XMLV - |
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章丽敏
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朱静
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李晶晶
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周雪
13372109301
| 产品名称： | CHO-INSR-1284 |
|---|---|
| 商品货号： | TS260463 |
| <strong>Cell line </strong>： | CHO-INSR-1284 |
| DSMZ no. ： | TS260463 |
| Species ： | confirmed as Cricetulus griseues (Chinese hamster) origin by COI DNA barcoding |
| Cell type ： | ovary cells |
| Origin ： | Flp-In derivate of CHO-K1 cell line (originated from an ovary biopsy of an adult Chinese hamster in 1957) which were transfected with the gene for human insulin receptor B (INSR) inserted in the vector pCDNA(TM)5-FRT-TO from Invitrogen |
| Reference(s) ： | 18190 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions ： | A, D |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid-like cells growing adherently in monolayers; image; image; image |
| Medium ： | 90% Ham´s F12 + 10% h.i. FBS + 1 mM L-glutamine + 0.3 mg/ml Hygromycin |
| Subculture ： | seed out 0.5-1 x 106 cells/80cm2; split 1:4 to 1:6 once or twice a week using accutase; after thawing viability may decrease and cells may need a few days to recover |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 8-10 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in PCR assay |
| Immunology ： | CD220 +; image |
| Viruses ： | PCR: XMLV - |
