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FAIK3-5
FAIK3-5
规格:
货期:
编号:TS260476
品牌:Testobio
产品名称: FAIK3-5
商品货号: TS260476
<strong>Cell line </strong>: FAIK3-5
DSMZ no. : TS260476
Species : confirmed as Mus musculus origin by COI DNA barcoding. STR profile is consistent with STR data of mouse C57/BL6
Cell type : kidney
Origin : REP (renal Epo-producing) cells from the kidney of adult transgenic female mice (B6D2;C57BL6N-Tg(EPO::Cre)1RHW x B6.Cg-Gt(ROSA)26Sor/J), immortalized with SV-40 large-T antigen. Cells are described to produce Epo under hypoxia (but no expression under normoxic conditions). Resistant to puromycin up to 0.5 µg/ml.
Reference(s) : 18189
Biosafety level : 1, GMO-S1
Risk assessment : genetically modified organism, according to German and European legislation allocated into group S1
Permissions and restrictions : A, D
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: flat, partially round, spindle and polygonal (fibroblastoid, telocyte-like) cells growing adherently in monolayers; image; image
Medium : 90-95% Dulbecco´s MEM + 5-10% h.i. FBS
Subculture : seed out 0.5-1 x 106 cells/80 cm2; split 1:5 to 1:10 every 3-4 days using trypsin/EDTA
Incubation : at 37 °C with 5% CO2, normal oxygen level
Doubling time : about 20-30 hours
Harvest : cell harvest of ca. 10 x 106 cells/80 cm2 flask
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Viruses : PCR: XMLV -
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FAIK3-5

  • 货号: TS260476
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  • 品牌 : TESTOBIO
产品名称: FAIK3-5
商品货号: TS260476
<strong>Cell line </strong>: FAIK3-5
DSMZ no. : TS260476
Species : confirmed as Mus musculus origin by COI DNA barcoding. STR profile is consistent with STR data of mouse C57/BL6
Cell type : kidney
Origin : REP (renal Epo-producing) cells from the kidney of adult transgenic female mice (B6D2;C57BL6N-Tg(EPO::Cre)1RHW x B6.Cg-Gt(ROSA)26Sor/J), immortalized with SV-40 large-T antigen. Cells are described to produce Epo under hypoxia (but no expression under normoxic conditions). Resistant to puromycin up to 0.5 µg/ml.
Reference(s) : 18189
Biosafety level : 1, GMO-S1
Risk assessment : genetically modified organism, according to German and European legislation allocated into group S1
Permissions and restrictions : A, D
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: flat, partially round, spindle and polygonal (fibroblastoid, telocyte-like) cells growing adherently in monolayers; image; image
Medium : 90-95% Dulbecco´s MEM + 5-10% h.i. FBS
Subculture : seed out 0.5-1 x 106 cells/80 cm2; split 1:5 to 1:10 every 3-4 days using trypsin/EDTA
Incubation : at 37 °C with 5% CO2, normal oxygen level
Doubling time : about 20-30 hours
Harvest : cell harvest of ca. 10 x 106 cells/80 cm2 flask
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in PCR assay
Viruses : PCR: XMLV -
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