| 产品名称: |
FAIK3-5 |
| 商品货号: |
TS260476 |
| <strong>Cell line </strong>: |
FAIK3-5 |
| DSMZ no. : |
TS260476 |
| Species : |
confirmed as Mus musculus origin by COI DNA barcoding. STR profile is consistent with STR data of mouse C57/BL6 |
| Cell type : |
kidney |
| Origin : |
REP (renal Epo-producing) cells from the kidney of adult transgenic female mice (B6D2;C57BL6N-Tg(EPO::Cre)1RHW x
B6.Cg-Gt(ROSA)26Sor/J), immortalized with SV-40 large-T antigen. Cells are described to produce Epo under hypoxia (but no expression under normoxic conditions). Resistant to puromycin up to 0.5 µg/ml. |
| Reference(s) : |
18189 |
| Biosafety level : |
1, GMO-S1 |
| Risk assessment : |
genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions : |
A, D |
| <strong> </strong>: |
DSMZ Scientific Data: |
| Morphology: |
flat, partially round, spindle and polygonal (fibroblastoid, telocyte-like) cells growing adherently in monolayers; image; image |
| Medium : |
90-95% Dulbecco´s MEM + 5-10% h.i. FBS |
| Subculture : |
seed out 0.5-1 x 106 cells/80 cm2;
split 1:5 to 1:10 every 3-4 days using trypsin/EDTA |
| Incubation : |
at 37 °C with 5% CO2, normal oxygen level |
| Doubling time : |
about 20-30 hours |
| Harvest : |
cell harvest of ca. 10 x 106 cells/80 cm2 flask |
| Storage : |
frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : |
negative in PCR assay |
| Viruses : |
PCR: XMLV - |